Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Deep sequencing identifies two genotypes and high viral genetic diversity of human pegivirus (GB virus C) in rural Ugandan patients
Journal of General Virology, Volume 94, No. PART 12, Year 2013
Notification
URL copied to clipboard!
Description
Human pegivirus (HPgV), formerly 'GB virus C' or 'hepatitis G virus', is a member of the genus Flavivirus (Flaviviridae) that has garnered significant attention due to its inhibition of HIV, including slowing disease progression and prolonging survival in HIV-infected patients. Currently, there are six proposed HPgV genotypes that have roughly distinct geographical distributions. Genotypes 2 and 3 are the most comprehensively characterized, whereas those genotypes occurring on the African continent, where HPgV prevalence is highest, are less well studied. Using deep sequencing methods, we identified complete coding HPgV sequences in four of 28 patients (14.3 %) in rural Uganda, east Africa. One of these sequences corresponds to genotype 1 and is the first complete genome of this genotype from east Africa. The remaining three sequences correspond to genotype 5, a genotype that was previously considered exclusively South African. All four positive samples were collected within a geographical area of less than 25 km2, showing that multiple HPgV genotypes co-circulate in this area. Analysis of intra-host viral genetic diversity revealed that total single-nucleotide polymorphism frequency was approximately tenfold lower in HPgV than in hepatitis C virus. Finally, one patient was co-infected with HPgV and HIV, which, in combination with the high prevalence of HIV, suggests that this region would be a useful locale to study the interactions and co-evolution of these viruses. © 2013 SGM.
Authors & Co-Authors
Ghai, Ria R.
Canada, Montreal
Université Mcgill
Sibley, Samuel D.
United States, Madison
University of Wisconsin-madison
Lauck, Michael
United States, Madison
University of Wisconsin-madison
Dinis, Jorge M.
United States, Madison
University of Wisconsin-madison
Bailey, Adam L.
United States, Madison
University of Wisconsin-madison
Chapman, Colin A.
Canada, Sainte-anne-de-bellevue
Mcgill University, Macdonald Campus
United States, New York
Wildlife Conservation Society
Omeja, Patrick Aria
Uganda, Kampala
Makerere University
Friedrich, Thomas C.
United States, Madison
University of Wisconsin-madison
United States, Madison
Wisconsin National Primate Research Center
O'Connor, David H.
United States, Madison
University of Wisconsin-madison
United States, Madison
University of Wisconsin School of Medicine and Public Health
Goldberg, Tony L.
United States, Madison
University of Wisconsin-madison
Uganda, Kampala
Makerere University
United States, Madison
Wisconsin National Primate Research Center
Statistics
Citations: 10
Authors: 10
Affiliations: 7
Identifiers
Doi:
10.1099/vir.0.055509-0
ISSN:
00221317
e-ISSN:
14652099
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases
Study Design
Cross Sectional Study
Study Locations
Multi-countries
Uganda