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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Capture immunoassay for ruminant tumor necrosis factor-α: comparison with bioassay
Veterinary Immunology and Immunopathology, Volume 35, No. 3-4, Year 1993
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Description
Monoclonal antibodies and IgG purified from rabbit polyclonal antiserum, raised against recombinant bovine tumor necrosis factor-alpha (TNF-α), have been employed in ELISA procedures to quantitate bovine TNF-α. These antibodies were potent in neutralizing the biological activity of recombinant as well as natural bovine TNF-α. The monoclonal antibodies were used as capture antibodies and were either passively adsorbed or covalently linked to ELISA plates. Polyclonal rabbit anti-TNF IgG was used as the detecting antibody in combination with a biotinylated anti-rabbit serum and a streptavidin-horseradish peroxidase conjugate. The detection limit for recombinant TNF-α medium was 10 pg ml-1 and in bovine or ovine serum was 35 pg ml-1. A good correlation was found between the ELISA and the WEHI-164 Clone 13 biologic assay when TNF-α was measured in medium containing serum or in serum. This capture ELISA was also capable of detecting ovine, but not porcine. TNF in supernatants from cultures of lipopolysaccharide-stimulated pulmonary alveolar macrophages. © 1993.
Authors & Co-Authors
Ellis, John A.
Canada, Saskatoon
Vaccine and Infectious Disease Organization - International Vaccine Centre
United States, Laramie
University of Wyoming
Godson, Dale
Canada, Saskatoon
Vaccine and Infectious Disease Organization - International Vaccine Centre
Campos, Manuel
Canada, Saskatoon
Vaccine and Infectious Disease Organization - International Vaccine Centre
Sileghem, Maarten R.
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Babiuk, Lorne A.
Canada, Saskatoon
Vaccine and Infectious Disease Organization - International Vaccine Centre
Statistics
Citations: 41
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1016/0165-2427(93)90040-B
ISSN:
01652427
Research Areas
Cancer