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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Detection of novel porcine bocaviruses in fecal samples of asymptomatic pigs in Cameroon
Infection, Genetics and Evolution, Volume 17, Year 2013
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Description
Improvements and widespread use of nucleic acid amplification and sequencing methods have led to the recognition of new virus diversity in various domestic animals, including pigs. In this study we utilized either virus species specific or broadly reactive PCR assays to describe the occurrence and genetic diversity of selected DNA viruses belonging to families Adenoviridae, Circoviridae, Anelloviridae and Parvoviridae in Cameroonian pigs. Fecal specimens were collected during spring of 2011. No adenoviruses, circoviruses and anelloviruses were detected, however, high prevalence and remarkable genetic diversity within the identified parvoviruses and, particularly, within bocaviruses was observed. PPV4 was the most prevalent virus (20%), followed by PBoV3 (18%), PBoV4 (18%), PBoV5 plus 6V/7V (16%), and PBoV1 plus PBoV2 (6%). The frequency of mixed infections with various combinations of these virus species reached 20%. Genetic analysis of the identified viruses showed that the capsid gene of PBoV1 and PBoV2 strains shared up to 91% and 94%. nt sequence similarities to reference PBoV1 and PBoV2 strains, respectively. The identified PBoV3 and PBoV4 strains shared ≤95% and ≤98%. nt identities with reference PBoV3 and PBoV4 strains, respectively, along the NS gene, whereas the PBoV5 strains shared 86%. nt identities with Hungarian and 87%. nt identities with Chinese PBoV5 strains along the capsid gene. In addition, a single PBoV5-like strain shared ≤71%. nt sequence identity with other PBoV5 strains. This is the first study to report evidence of the circulation of bocaviruses in Africa and contributes to our understanding of the impact of globalization on the dispersal of new and emerging viruses. © 2013.
Authors & Co-Authors
Ndze, Valentine Ngum
Cameroon, Yaounde
Université de Yaoundé I
Cadar, Dániel
Romania, Cluj Napoca
Universitatea de Stiinte Agricole si Medicina Veterinara Din Cluj-napoca
Cságola, Attila
Hungary, Godollo
Hungarian University of Agriculture and Life Sciences
Kisfali, Péter
Hungary, Pecs
University of Pécs Medical School
Kovács, Eszter
Hungary, Budapest
Magyar Tudomanyos Akademia
Farkas, Szilvia
Hungary, Budapest
Magyar Tudomanyos Akademia
Ngu, Akum Felix
Cameroon
Regional Hospital Laboratory
Esona, Mathew Dioh
United States, Atlanta
National Center for Immunization and Respiratory Diseases
Dán, Ádám
Hungary, Budapest
National Food Chain Safety Office
Tuboly, Tamás
Hungary, Godollo
Hungarian University of Agriculture and Life Sciences
Bányai, Krisztián
Hungary, Budapest
Magyar Tudomanyos Akademia
Statistics
Citations: 28
Authors: 11
Affiliations: 8
Identifiers
Doi:
10.1016/j.meegid.2013.03.006
ISSN:
15671348
e-ISSN:
15677257
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study
Study Locations
Cameroon