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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Detection of Streptococcus pneumoniae and Haemophilus influenzae Type B by Real-Time PCR from Dried Blood Spot Samples among Children with Pneumonia: A Useful Approach for Developing Countries
PLoS ONE, Volume 8, No. 10, Article e76970, Year 2013
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Description
Background:Dried blood spot (DBS) is a reliable blood collection method for storing samples at room temperature and easily transporting them. We have previously validated a Real-Time PCR for detection of Streptococcus pneumoniae in DBS. The objective of this study was to apply this methodology for the diagnosis of S. pneumoniae and Haemophilus influenzae b (Hib) in DBS samples of children with pneumonia admitted to two hospitals in Mozambique and Morocco.Methods:Ply and wzg genes of S. pneumoniae and bexA gene of Hib, were used as targets of Real-Time PCR. 329 DBS samples of children hospitalized with clinical diagnosis of pneumonia were tested.Results:Real-Time PCR in DBS allowed for a significant increase in microbiological diagnosis of S. pneumoniae and Hib. When performing blood bacterial culture, only ten isolates of S. pneumoniae and none of Hib were detected (3·0% positivity rate, IC95% 1·4-5·5%). Real-Time PCR from DBS samples increased the detection yield by 4x fold, as 30 S. pneumoniae and 11 Hib cases were detected (12·4% positivity rate, IC95% 9·0-16·5%; P<0·001).Conclusion:Real-Time PCR applied in DBS may be a valuable tool for improving diagnosis and surveillance of pneumonia caused by S. pneumoniae or Hib in developing countries. © 2013 Selva et al.
Authors & Co-Authors
Selva, Laura
Spain, Esplugues de Llobregat
Hospital Sant Joan de Déu
Benmessaoud, Rachid
Spain, Barcelona
Instituto de Salud Global de Barcelona
Morocco, Agdal Rabat
Fundació Clínic Per a la Recerca Biomèdica
Lanaspa, Miguel
Spain, Barcelona
Instituto de Salud Global de Barcelona
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
Jroundi, Imane
Spain, Barcelona
Instituto de Salud Global de Barcelona
Morocco, Agdal Rabat
Ministry of Health, Morocco
Moraleda, Cinta
Spain, Barcelona
Instituto de Salud Global de Barcelona
Morocco, Agdal Rabat
Fundació Clínic Per a la Recerca Biomèdica
Acácio, Sozinho
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
Iñigo, Melania
Spain, Esplugues de Llobregat
Hospital Sant Joan de Déu
Bastiani, Alien
Spain, Esplugues de Llobregat
Hospital Sant Joan de Déu
Monsonis, Manuel
Spain, Esplugues de Llobregat
Hospital Sant Joan de Déu
Pallares, Roman
Spain, Barcelona
Universitat de Barcelona
Bassat, Quique
Spain, Barcelona
Instituto de Salud Global de Barcelona
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
Muñoz-Almagro, Carmen
Spain, Esplugues de Llobregat
Hospital Sant Joan de Déu
Statistics
Citations: 25
Authors: 12
Affiliations: 6
Identifiers
Doi:
10.1371/journal.pone.0076970
e-ISSN:
19326203
Research Areas
Genetics And Genomics
Maternal And Child Health
Study Locations
Morocco
Mozambique