Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Comparison of real-time PCR assays for detection of pathogenic Leptospira spp. in blood and identification of variations in target sequences
Journal of Clinical Microbiology, Volume 49, No. 6, Year 2011
Notification
URL copied to clipboard!
Description
Leptospirosis is considered an underdiagnosed disease. Although several PCR-based methods are currently in use, there is little information on their comparability. In this study, four quantitative real-time PCR (qPCR) assays (SYBR green and TaqMan chemistries) targeting the secY, lfb1, and lipL32 genes were evaluated as diagnostic assays. In our hands, these assays can detect between 102 and 103 bacteria/ml of pure culture, whole-blood, plasma, and serum samples. In three independent experiments, we found a slightly higher sensitivity of the PCR assays in plasma than in whole blood and serum. We also evaluated the specificity of the PCR assays on reference Leptospira strains, including newly described Leptospira species, and clinical isolates. No amplification was detected for DNA obtained from saprophytic or intermediate Leptospira species. However, among the pathogens, we identified sequence polymorphisms in target genes that result in primer and probe mismatches and affect qPCR assay performance. In conclusion, most of these assays are sensitive and specific tools for routine diagnosis of leptospirosis. However, it is important to continually evaluate and, if necessary, modify the primers and/or probes used to ensure effective detection of the circulating Leptospira isolates. Copyright © 2011, American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3122738/bin/supp_49_6_2154__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3122738/bin/supp_49_6_2154__Supplementaldata25nov.zip
Authors & Co-Authors
Bourhy, Pascale
France, Paris
Institut Pasteur, Paris
Bremont, S.
France, Paris
Institut Pasteur, Paris
Zinini, Farida
France, Paris
Institut Pasteur, Paris
Giry, Claude
Mayotte, Mamoudzou
Centre Hospitalier de Mayotte
Picardeau, Mathieu
France, Paris
Institut Pasteur, Paris
Statistics
Citations: 136
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1128/JCM.02452-10
ISSN:
00951137
e-ISSN:
1098660X
Research Areas
Genetics And Genomics
Study Approach
Quantitative