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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Antimicrobial peptides and protease inhibitors in the skin secretions of the crawfish frog, Rana areolata
Biochimica et Biophysica Acta - Proteins and Proteomics, Volume 1601, No. 1, Year 2002
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Description
The dorsal skin of the crawfish frog, Rana areolata, is associated with numerous prominent granular glands. Proteomic analysis of electrically stimulated skin secretions from these glands enabled the identification and characterization of eight peptides with antimicrobial and hemolytic activity belonging to the previously identified brevinin-1, temporin-1, palustrin-2, palustrin-3, esculentin-1 (two peptides), and ranatuerin-2 (two peptides) families. The primary structures of the peptides were consistent with a close phylogenetic relationship between R. areolata and the pickerel frog, Rana palustris. Three structurally related cationic, cysteine-containing peptides were identified that show sequence similarity to peptide Leucine-Arginine, a peptide with immunomodulatory and histamine-releasing properties from the skin of the northern leopard frog, Rana pipiens. The skin secretions contained a 61-amino-acid-residue peptide that inhibited porcine trypsin and possessed a 10-cysteine-residue motif that is characteristic of a protease inhibitor previously isolated from the parasitic nematode, Ascaris suum. A 48-amino-acid-residue protein containing eight cysteine residues in the whey acidic protein (WAP) motif, characteristic of elafin (skin-derived antileukoproteinase) and secretory leukocyte protease inhibitor, was also isolated. The data suggest that protease inhibitors in skin secretions may play a role complementary to cationic, amphipathic α-helical peptides in protecting anurans from invasions by microorganisms. © 2002 Elsevier Science B.V. All rights reserved.
Authors & Co-Authors
Ali, Mohammed Hasan M.
United States, Omaha
Creighton University School of Medicine
Lips, Karen R.
United States, Carbondale
Southern Illinois University Carbondale
Knoop, Floyd C.
United States, Omaha
Creighton University School of Medicine
Fritzsch, Bernd
United States, Omaha
Creighton University School of Medicine
Miller, Caroline A.
United States, Omaha
Creighton University School of Medicine
Conlon, John Michael
United States, Omaha
Creighton University School of Medicine
United Arab Emirates, Al Ain
United Arab Emirates University
Statistics
Citations: 104
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/S1570-9639(02)00432-6
ISSN:
15709639