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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Nitrative DNA damage and Oct3/4 expression in urinary bladder cancer with Schistosoma haematobium infection
Biochemical and Biophysical Research Communications, Volume 414, No. 2, Year 2011
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Description
To investigate whether mutant stem cells participate in inflammation-related carcinogenesis, we performed immunohistochemical analysis to examine nitrative and oxidative DNA lesions (8-nitroguanine and 8-oxodG) and a stem cell marker Oct3/4 in bladder tissues obtained from cystitis and bladder cancer patients infected with Schistosoma haematobium (S. haematobium). We also detected the expression of nuclear factor-κB (NF-κB) and inducible nitric oxide synthase (iNOS), which lead to 8-nitroguanine formation. The staining intensity of 8-nitroguanine and 8-oxodG was significantly higher in bladder cancer and cystitis tissues than in normal tissues. iNOS expression was colocalized with NF-κB in 8-nitroguanine-positive tumor cells from bladder cancer patients. Oct3/4 expression was significantly increased in cells from S. haematobium-associated bladder cancer tissues in comparison to normal bladder and cancer tissues without infection. Oct3/4 was also expressed in epithelial cells of cystitis patients. Moreover, 8-nitroguanine was formed in Oct3/4-positive stem cells in S. haematobium-associated cystitis and cancer tissues. In conclusion, inflammation by S. haematobium infection may increase the number of mutant stem cells, in which iNOS-dependent DNA damage occurs via NF-κB activation, leading to tumor development. © 2011 Elsevier Inc.
Authors & Co-Authors
Ma, Ning
Japan, Suzuka
Suzuka University of Medical Science
Thanan, Raynoo
Japan, Suzuka
Suzuka University of Medical Science
Japan, Tsu
Mie University Graduate School of Medicine
Kobayashi, Hatasu
Japan, Tsu
Mie University Graduate School of Medicine
Hammam, Olfat Ali
Egypt, Giza
Theodor Bilharz Research Institute
Wishahi, Mohamed
Egypt, Giza
Theodor Bilharz Research Institute
LeitHy, Tarek El
Egypt, Giza
Theodor Bilharz Research Institute
Hiraku, Yusuke
Japan, Tsu
Mie University Graduate School of Medicine
Amro, El Karef
Japan, Tsu
Mie University Graduate School of Medicine
Egypt, Mansoura
Faculty of Medicine
Oikawa, Shinji
Japan, Tsu
Mie University Graduate School of Medicine
Ohnishi, Shiho
Japan, Suzuka
Suzuka University of Medical Science
Murata, Mairiko
Japan, Tsu
Mie University Graduate School of Medicine
Kawanishi, Shousuke
Japan, Suzuka
Suzuka University of Medical Science
Japan, Tsu
Mie University Graduate School of Medicine
Statistics
Citations: 50
Authors: 12
Affiliations: 4
Identifiers
Doi:
10.1016/j.bbrc.2011.09.073
ISSN:
0006291X
e-ISSN:
10902104
Research Areas
Cancer
Genetics And Genomics