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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Systems-based approaches enable identification of gene targets which improve the flavour profile of low-ethanol wine yeast strains
Metabolic Engineering, Volume 49, Year 2018
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Description
Metabolic engineering has been vital to the development of industrial microbes such as the yeast Saccharomyces cerevisiae. However, sequential rounds of modification are often needed to achieve particular industrial design targets. Systems biology approaches can aid in identifying genetic targets for modification through providing an integrated view of cellular physiology. Recently, research into the generation of commercial yeasts that can produce reduced-ethanol wines has resulted in metabolically-engineered strains of S. cerevisiae that are less efficient at producing ethanol from sugar. However, these modifications led to the concomitant production of off-flavour by-products. A combination of transcriptomics, proteomics and metabolomics was therefore used to investigate the physiological changes occurring in an engineered low-ethanol yeast strain during alcoholic fermentation. Integration of ‘omics data identified several metabolic reactions, including those related to the pyruvate node and redox homeostasis, as being significantly affected by the low-ethanol engineering methodology, and highlighted acetaldehyde and 2,4,5-trimethyl-1,3-dioxolane as the main off-flavour compounds. Gene remediation strategies were then successfully applied to decrease the formation of these by-products, while maintaining the ‘low-alcohol’ phenotype. The data generated from this comprehensive systems-based study will inform wine yeast strain development programmes, which, in turn, could potentially play an important role in assisting winemakers in their endeavour to produce low-alcohol wines with desirable flavour profiles. © 2018 International Metabolic Engineering Society
Authors & Co-Authors
Hodson, Mark P.
Australia, Brisbane
The University of Queensland
Australia, Sydney
Victor Chang Cardiac Research Institute
Pretorius, Isak S.
Australia, Sydney
Macquarie University
Roessner, Ute
Australia, Melbourne
University of Melbourne
Wilkins, Marc R.
Australia, Sydney
Unsw Sydney
Statistics
Citations: 16
Authors: 4
Affiliations: 9
Identifiers
Doi:
10.1016/j.ymben.2018.08.006
ISSN:
10967176
Research Areas
Genetics And Genomics
Noncommunicable Diseases
Substance Abuse