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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
engineering
Mimicking filtration and transport of rotavirus and adenovirus in sand media using DNA-labeled, protein-coated silica nanoparticles
Water Research, Volume 62, Year 2014
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Description
Rotavirus (RoV) and adenovirus (AdV) are important viral pathogens for the risk analysis of drinking water. Despite this, little is known about their retention and transport behaviors in porous media due to a lack of representative surrogates. We developed RoV and AdV surrogates by covalently coupling 70-nm sized silica nanoparticles with specific proteins and a DNA marker for sensitive detection. Filtration experiments using beach sand columns demonstrated the similarity of the surrogates' concentrations, filtration efficiencies and attachment kinetics to those of the target viruses. The surrogates showed the same magnitude of concentration reduction as the viruses. Conversely, MS2 phage (a traditional virus model) over-predicted concentrations of AdV and RoV by 1- and 2-orders of magnitude respectively. The surrogates remained stable in size, surface charge and DNA concentration for at least one year. They can be easily and rapidly detected down to a single particle. Preliminary tests suggest that they were readily detectable in a number of environmental waters and treated effluent. With up-scaling validation in pilot trials, the surrogates developed here could be a cost-effective new tool for studying virus retention and transport in porous media. Examples include assessing filter efficacy in water and wastewater treatment, tracking virus migration in groundwater after effluent land disposal, and establishing safe setback distances for groundwater protection. © 2014 Elsevier Ltd.
Authors & Co-Authors
Pang, Liping
New Zealand, Christchurch
Esr - Christchurch Science Centre
Farkas, Kata
New Zealand, Christchurch
Esr - Christchurch Science Centre
New Zealand, Christchurch
University of Canterbury
Bennett, Grant
New Zealand, Christchurch
Bennett Scientific
New Zealand, Lincoln
Lincoln University
Varsani, Arvind
New Zealand, Christchurch
University of Canterbury
South Africa, Cape Town
University of Cape Town
Easingwood, Richard A.
New Zealand, Dunedin
University of Otago
Tilley, Richard
New Zealand, Wellington
Victoria University of Wellington
Nowostawska, Urszula
New Zealand, Dunedin
University of Otago
Lin, Susan
New Zealand, Christchurch
Esr - Christchurch Science Centre
Statistics
Citations: 43
Authors: 8
Affiliations: 7
Identifiers
Doi:
10.1016/j.watres.2014.05.055
ISSN:
00431354
e-ISSN:
18792448
Research Areas
Environmental
Genetics And Genomics