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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Inhibition of maize streak virus (MSV) replication by transient and transgenic expression of MSV replication-associated protein mutants
Journal of General Virology, Volume 88, No. 1, Year 2007
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Description
Maize streak disease is a severe agricultural problem in Africa and the development of maize genotypes resistant to the causal agent, Maize streak virus (MSV), is a priority. A transgenic approach to engineering MSV-resistant maize was developed and tested in this study. A pathogen-derived resistance strategy was adopted by using targeted deletions and nucleotide-substitution mutants of the multifunctional MSV replication-associated protein gene (rep). Various rep gene constructs were tested for their efficacy in limiting replication of wild-type MSV by co-bombardment of maize suspension cells together with an infectious genomic clone of MSV and assaying replicative forms of DNA by quantitative PCR. Digitaria sanguinalis, an MSV-sensitive grass species used as a model monocot, was then transformed with constructs that had inhibited virus replication in the transient-expression system. Challenge experiments using leafhopper-transmitted MSV indicated significant MSV resistance - from highly resistant to immune - in regenerated transgenic D. sanguinalis lines. Whereas regenerated lines containing a mutated full-length rep gene displayed developmental and growth defects, those containing a truncated rep gene both were fertile and displayed no growth defects, making the truncated gene a suitable candidate for the development of transgenic MSV-resistant maize. © 2007 SGM.
Authors & Co-Authors
Shepherd, Dionne Natalie
South Africa, Cape Town
University of Cape Town
Mangwende, Tichaona
South Africa, Cape Town
University of Cape Town
Martin, Darren Patrick
South Africa, Cape Town
University of Cape Town
Bezuidenhout, Marion E.
South Africa, Cape Town
University of Cape Town
Thomson, Jennifer Ann
South Africa, Cape Town
University of Cape Town
Rybicki, Edward P.
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 41
Authors: 6
Affiliations: 1
Identifiers
Doi:
10.1099/vir.0.82338-0
ISSN:
00221317
Research Areas
Genetics And Genomics
Study Approach
Quantitative