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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Whole Genome Sequencing of Field Isolates Reveals a Common Duplication of the Duffy Binding Protein Gene in Malagasy Plasmodium vivax Strains
PLoS Neglected Tropical Diseases, Volume 7, No. 11, Article e2489, Year 2013
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Description
Background:Plasmodium vivax is the most prevalent human malaria parasite, causing serious public health problems in malaria-endemic countries. Until recently the Duffy-negative blood group phenotype was considered to confer resistance to vivax malaria for most African ethnicities. We and others have reported that P. vivax strains in African countries from Madagascar to Mauritania display capacity to cause clinical vivax malaria in Duffy-negative people. New insights must now explain Duffy-independent P. vivax invasion of human erythrocytes.Methods/Principal Findings:Through recent whole genome sequencing we obtained ≥70× coverage of the P. vivax genome from five field-isolates, resulting in ≥93% of the Sal I reference sequenced at coverage greater than 20×. Combined with sequences from one additional Malagasy field isolate and from five monkey-adapted strains, we describe here identification of DNA sequence rearrangements in the P. vivax genome, including discovery of a duplication of the P. vivax Duffy binding protein (PvDBP) gene. A survey of Malagasy patients infected with P. vivax showed that the PvDBP duplication was present in numerous locations in Madagascar and found in over 50% of infected patients evaluated. Extended geographic surveys showed that the PvDBP duplication was detected frequently in vivax patients living in East Africa and in some residents of non-African P. vivax-endemic countries. Additionally, the PvDBP duplication was observed in travelers seeking treatment of vivax malaria upon returning home. PvDBP duplication prevalence was highest in west-central Madagascar sites where the highest frequencies of P. vivax-infected, Duffy-negative people were reported.Conclusions/Significance:The highly conserved nature of the sequence involved in the PvDBP duplication suggests that it has occurred in a recent evolutionary time frame. These data suggest that PvDBP, a merozoite surface protein involved in red cell adhesion is rapidly evolving, possibly in response to constraints imposed by erythrocyte Duffy negativity in some human populations. © 2013 Menard et al.
Available Materials
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https://efashare.b-cdn.net/share/pmc/articles/PMC3836732/bin/pntd.0002489.s007.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3836732/bin/pntd.0002489.s008.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3836732/bin/pntd.0002489.s009.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3836732/bin/pntd.0002489.s010.xlsx
Authors & Co-Authors
Menard, Didier
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Chan, E. R.
United States, Cleveland
Cleveland Clinic Foundation
Benedet, Christophe
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Ratsimbasoa, Arsène C.
Madagascar, Antananarivo
Direction de la Lutte Contre Les Maladies Infectieuses
Kim, Saorin
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Chim, Pheaktra
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Do, Catherine
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Witkowski, Benoît
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Durand, Rémy
France, Bobigny
Hopital Avicenne
Thellier, Marc
France, Paris
Hôpital Universitaire Pitié Salpêtrière
Severini, Carlo
Italy, Rome
Istituto Superiore Di Sanita
Legrand, Eric
French Guiana, Cayenne
Institut Pasteur de la Guyane
Musset, Lise
French Guiana, Cayenne
Institut Pasteur de la Guyane
Nour, Bakri Y.M.
Sudan, Wad Medani
University of Gezira
Mercereau-Puijalon, Odile
France, Paris
Institut Pasteur, Paris
Serre, David
United States, Cleveland
Cleveland Clinic Foundation
Zimmerman, Peter A.
United States, Cleveland
Case School of Medicine
Statistics
Citations: 110
Authors: 17
Affiliations: 10
Identifiers
Doi:
10.1371/journal.pntd.0002489
ISSN:
19352727
e-ISSN:
19352735
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Infectious Diseases
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Multi-countries
Madagascar
Mauritania