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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Spread of Influenza A(H1N1) oseltamivir-resistant viruses in Africa in 2008 confirmed by multiple introductions in Senegal
BMC Infectious Diseases, Volume 13, No. 1, Article 106, Year 2013
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Description
Background: Among Influenza neuraminidase inhibitors (NAIs), oseltamivir corresponds to the most widely used agent to treat influenza disease. However since 2001, several cases of resistance to NAIs have been reported for circulating seasonal A(H1N1) Influenza viruses. A direct resistance mechanism may be invoked, involving critical mutations in the viral NA gene that prevent the drug binding to its target. Same phenomenon is reported for adamantanes drugs and mutations in the M2 channel protein gene of Influenza viruses. Methods: Reverse-Transcription/Restriction Fragment Length Polymorphism (RT-PCR/RFLP) method, phenotypic testing for oseltamivir resistance, and sequencing of NA, HA and M2 genes were used in this study. Phylogenetic analyses were performed using BioEdit and Mega 5 softwares for alignment of sequences and phylogenetic trees building respectively. Results: Using a simple RT-PCR/RFLP method, we found that the 86 seasonal A(H1N1) isolates from 2008 bear the oseltamivir resistance-associated mutation (H274Y) in the NA gene. In contrast all isolates isolated in Senegal in 2007 were sensitive to oseltamivir. These results were first confirmed by finding high IC50 values using a phenotypic testing for oseltamivir resistance, and secondly by sequencing the whole NA gene. Regarding M2 gene, no mutation associated to adamantanes resistance was characterized of the isolates. Conclusions: The present work provides evidence of circulation of drug-resistant seasonal A(H1N1) viruses during the 2008 influenza season (July to September) in Senegal. The results are in favor of multiple introductions of oseltamivir resistant viruses (ORV) A(H1N1) in Senegal.Phylogenetic analyses of isolates with complete sequences of N1 and HA1 genes showed that they belong to clade 2B and suggest sequential introductions in Africa. © 2013 Dia et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Dia, Ndongo D.
Senegal, Dakar
Institut Pasteur de Dakar
Niang, Mbayame N’Diaye D.
Senegal, Dakar
Institut Pasteur de Dakar
Diadhiou, Saadiya A.
Senegal, Dakar
Ministère de la Santé et de la Prévention
Goudiaby, Déborah G.
Senegal, Dakar
Institut Pasteur de Dakar
Faye, Abdourahmane
Senegal, Dakar
Institut Pasteur de Dakar
Kiori, Davy Evrard
Senegal, Dakar
Institut Pasteur de Dakar
Bâ, Mady
Senegal, Dakar
Ministère de la Santé et de la Prévention
Michel, Rémy
Senegal, Dakar
Institut Pasteur de Dakar
Diop, Ousmane Madiagne
Senegal, Dakar
Institut Pasteur de Dakar
Statistics
Citations: 9
Authors: 9
Affiliations: 2
Identifiers
Doi:
10.1186/1471-2334-13-106
e-ISSN:
14712334
Research Areas
Cancer
Genetics And Genomics
Study Locations
Senegal