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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
"Ultrathin" DSAEK tissue prepared with a low-pulse energy, high-frequency femtosecond laser
Cornea, Volume 32, No. 1, Year 2013
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Description
PURPOSE: To evaluate the endothelial cell survival and stromal bed quality when creating deep stromal cuts with a low-pulse energy, high-frequency femtosecond laser to produce "ultrathin" tissue for Descemet stripping automated endothelial keratoplasty. METHODS: Seventeen corneas were used for this study. Five corneas were cut with the laser at a depth of 420 to 500 μm to produce a tissue thickness of approximately ≤70 μm. Five corneas served as an uncut comparison group. Vital dye staining and computer digitized planimetry analysis were performed on these corneas. The 7 remaining corneas were cut for scanning electron microscopy evaluation. RESULTS: The mean central posterior stromal thickness of cut corneas was 60.6 μm (range, 43-72 μm). Endothelial cell damage in cut and comparison corneas was 3.92% ± 2.22% (range, 1.71%-6.51%) and 4.15% ± 2.64% (range, 1.21%-7.01%), respectively (P = 0.887). Low-magnification (×12) scanning electron microscopy revealed a somewhat irregular-appearing surface with concentric rings peripherally. Qualitative grading of higher magnification (×50) central images resulted in an average score of 2.56 (between smooth and rough). CONCLUSIONS: Ultrathin tissue for Descemet stripping automated endothelial keratoplasty can be safely prepared with minimal endothelial cell damage using a low-pulse energy, high-frequency femtosecond laser; however, the resulting stromal surface quality may not be optimal with this technique. Copyright © 2012 by Lippincott Williams & Wilkins.
Authors & Co-Authors
Phillips, Paul Mathew
United States, Pittsburgh
Sightline Ophthalmic Associates
Phillips, Louis J.
United States, Pittsburgh
Sightline Ophthalmic Associates
Saad, Hisham A.
Egypt, Tanta
Faculty of Medicine
Terry, Mark A.
United States, Portland
Devers Eye Institute
United States, Portland
Lions Visiongift
Stolz, Donna Beer
United States, Pittsburgh
University of Pittsburgh School of Medicine
Stoeger, Christopher G.
United States, Portland
Lions Visiongift
Franks, Jonathan M.
United States, Pittsburgh
University of Pittsburgh School of Medicine
Davis-Boozer, David D.
United States, Portland
Lions Visiongift
Statistics
Citations: 43
Authors: 8
Affiliations: 5
Identifiers
Doi:
10.1097/ICO.0b013e31825c72dc
e-ISSN:
15364798
Study Design
Quasi Experimental Study
Study Approach
Qualitative