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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification
Theriogenology, Volume 62, No. 3-4, Year 2004
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Description
The objectives of this study were to identify an improved in vitro cell-free embryo culture system and to compare post-warming development of in vitro produced (IVP) bovine embryos following vitrification versus slow freezing. In Experiment 1, non-selected presumptive zygotes were randomly allocated to four medium treatments without co-culture: (1) SOF+5% FCS for 9 days; (2) KSOM+0.1% BSA for 4 days and then KSOM+1% BSA to Day 9; (3) SOF+5% FCS for 4 days and then KSOM+1% BSA to Day 9; and (4) KSOM+0.1% BSA for 4 days and then SOF+5% FCS to Day 9. Treatment 4 (sequential KSOM-SOF culture system) improved (P>0.05) morulae (47%), early blastocysts (26%), Day-7 blastocysts (36%), cell numbers, as well as total hatching rate (79%) compared to KSOM alone (Treatment 2). Embryos cultured in KSOM+BSA alone developed slowly and most of them hatched late on Day 9, compared to other treatments. In Experiment 2, the sequential KSOM-SOF culture system was used and Day-7 blastocysts were subjected to following cryopreservation comparison: (1) vitrification (VS3a, 6.5 M glycerol); or (2) slow freezing (1.36 M glycerol). Warmed embryos were cultured in SOF with 7.5% FCS. Higher embryo development and hatching rates (P<0.05) were obtained by vitrification at 6 h (71%), 24 h (64%), and 48 h (60%) post-warming compared to slow freezing (48, 40, and 31%, respectively). Following transfer of vitrified embryos to synchronized recipients, a 30% pregnancy rate was obtained. In conclusion, replacing KSOM with SOF after 4 days of culture produced better quality blastocysts. Vitrification using VS3a may be used more effectively to cryopreserve in vitro produced embryos than the conventional slow freezing method. © 2003 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Nedambale, Tshimangadzo Lucky
United States, Storrs
University of Connecticut
Dinnyés, András
Hungary, Godollo
Hungarian University of Agriculture and Life Sciences
Groen, W.
Unknown Affiliation
Dobrinsky, John R.
United States, Beltsville
Usda Ars Beltsville Agricultural Research Center
Tian, Xiuchun (Cindy)
United States, Storrs
University of Connecticut
Yang, Xiangzhong Jerry
United States, Storrs
University of Connecticut
Statistics
Citations: 71
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/j.theriogenology.2003.10.020
ISSN:
0093691X
Research Areas
Maternal And Child Health
Sexual And Reproductive Health
Study Approach
Quantitative