Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Distinct patterns of blood-stage parasite antigens detected by plasma IgG subclasses from individuals with different level of exposure to Plasmodium falciparum infections
Malaria Journal, Volume 9, No. 1, Article 296, Year 2010
Notification
URL copied to clipboard!
Description
Background. In endemic regions naturally acquired immunity against Plasmodium falciparum develops as a function of age and exposure to parasite infections and is known to be mediated by IgG. The targets of protective antibodies remain to be fully defined. Several immunoepidemiological studies have indicated an association of cytophilic anti-parasite IgG with protection against malaria. It has been hypothesized that the initial antibody responses against parasite antigens upon first few Plasmodium falciparum infections is dominated by non-protective IgG2/IgG4 and IgM antibodies, which then gradually develop into protective response dominated by cytophilic IgG1 and IgG3 antibodies. Methods. Naturally occurring IgG antibodies against P. falciparum blood-stage antigens were analysed from plasma samples collected from four groups of individuals differing in age and level of exposure to P. falciparum infections. Western Blot profiling of blood-stage parasite antigens displaying reactivity with individual plasma samples in terms of their subclass specificities was conducted. Parasite antigens detected by IgG were grouped based on their apparent molecular sizes resolved by SDS-PAGE as high molecular weight ( 70 kDa) or low molecular weight (< 70 kDa). The number of discernable low molecular weight parasite antigens detected by different IgG subclass antibodies from each plasma sample was recorded. Using Wilcoxons rank sum test these reactivities were compared amongst groups of individuals with different levels of exposure to P. falciparum infections. Results. IgG4 and IgM antibodies in plasma samples from all groups detected very few parasite antigens. IgG2 antibodies from all groups detected a common pattern of high molecular weight parasite antigens. Cytophilic IgG subclasses in plasma samples from individuals with higher levels of exposure to P. falciparum infections distinctly detected higher numbers of low molecular weight parasite antigens. Conclusions. In the present study, there was no evidence for switching of antibody responses from non-cytophilic to cytophilic subclasses against blood-stage parasite antigens as a likely mechanism for induction of protective immunity against malaria. © 2010 Olesen et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Olesen, Cathrine Holm
Denmark, Copenhagen
Statens Serum Institut
Denmark, Bagsvard
Novo Nordisk A/s
Brahimi, Karima
France, Paris
Institut Pasteur, Paris
Vandahl, Brian
Denmark, Copenhagen
Statens Serum Institut
Denmark, Bagsvard
Novo Nordisk A/s
Lousada-Dietrich, Susana
Denmark, Copenhagen
Københavns Universitet
Jogdand, Prajakta S.
Denmark, Copenhagen
Københavns Universitet
Vestergaard, Lasse Skafte
Denmark, Copenhagen
Københavns Universitet
Denmark, Copenhagen
Statens Serum Institut
Dodoo, Daniel K.
Ghana, Accra
Noguchi Memorial Institute for Medical Research
Højrup, Peter
Denmark, Odense
Syddansk Universitet
Christiansen, Michael
Denmark, Copenhagen
Statens Serum Institut
Larsen, Severin Olesen
Denmark, Copenhagen
Statens Serum Institut
Singh, Subhash N.
Denmark, Copenhagen
Københavns Universitet
India, Srinagar
Indian Institute of Integrative Medicine, Srinagar
Theisen, Michael
Denmark, Copenhagen
Statens Serum Institut
Denmark, Copenhagen
Københavns Universitet
Statistics
Citations: 16
Authors: 12
Affiliations: 7
Identifiers
Doi:
10.1186/1475-2875-9-296
e-ISSN:
14752875
Research Areas
Infectious Diseases