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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Identification of novel transcription factors regulating secondary cell wall formation in Arabidopsis
Frontiers in Plant Science, Volume 4, No. JUN, Article 189, Year 2013
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Description
The presence of lignin in secondary cell walls (SCW) is a major factor preventing hydrolytic enzymes from gaining access to cellulose, thereby limiting the saccharification potential of plant biomass. To understand how lignification is regulated is a prerequisite for selecting plant biomass better adapted to bioethanol production. Because transcriptional regulation is a major mechanism controlling the expression of genes involved in lignin biosynthesis, our aim was to identify novel transcription factors (TFs) dictating lignin profiles in the model plant Arabidopsis. To this end, we have developed a post-genomic approach by combining four independent in-house SCW-related transcriptome datasets obtained from (1) the fiber cell wall-deficient wat1 Arabidopsis mutant, (2) Arabidopsis lines over-expressing either the master regulatory activator EgMYB2 or (3) the repressor EgMYB1 and finally (4) Arabidopsis orthologs of Eucalyptus xylem-expressed genes. This allowed us to identify 502 up- or down-regulated TFs. We preferentially selected those present in more than one dataset and further analyzed their in silico expression patterns as an additional selection criteria. This selection process led to 80 candidates. Notably, 16 of them were already proven to regulate SCW formation, thereby validating the overall strategy. Then, we phenotyped 43 corresponding mutant lines focusing on histological observations of xylem and interfascicular fibers. This phenotypic screen revealed six mutant lines exhibiting altered lignification patterns. Two of them [Bel-like HomeoBox6 (blh6) and a zinc finger TF] presented hypolignified SCW. Three others (myb52, myb-like TF, hb5) showed hyperlignified SCW whereas the last one (hb15) showed ectopic lignification. In addition, our meta-analyses highlighted a reservoir of new potential regulators adding to the gene network regulating SCW but also opening new avenues to ultimately improve SCW composition for biofuel production. © 2013 Cassan-Wang, Goué, Saidi, Legay, Sivadon, Goffner and Grima-Pettenati.
Authors & Co-Authors
Cassan-Wang, Hua
France, Toulouse
Université Toulouse Iii - Paul Sabatier
Goué, Nadia
France, Toulouse
Université Toulouse Iii - Paul Sabatier
Saidi, Mohammed Najib
France, Toulouse
Université Toulouse Iii - Paul Sabatier
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Legay, Sylvain
France, Toulouse
Université Toulouse Iii - Paul Sabatier
France, Versailles
Institut Jean-pierre Bourgin
Sivadon, Pierre
France, Toulouse
Université Toulouse Iii - Paul Sabatier
France, Pau
Universite de Pau et Des Pays de L'adour
Goffner, Deborah
France, Toulouse
Université Toulouse Iii - Paul Sabatier
Senegal, Dakar
Unité Mixte Internationale Environnement, Santé, Sociétés
Grima-Pettenati, Jacqueline
France, Toulouse
Université Toulouse Iii - Paul Sabatier
Statistics
Citations: 81
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.3389/fpls.2013.00189
e-ISSN:
1664462X
Research Areas
Genetics And Genomics
Health System And Policy