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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Novel application of a whole blood intracellular cytokine detection assay to quantitate specific T-cell frequency in field studies
Journal of Immunological Methods, Volume 291, No. 1-2, Year 2004
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Description
We optimized a whole blood intracellular cytokine assay to quantitate the frequency of specific CD4 + and CD8 + T cells in small volumes of whole blood from infants from developing countries. The assay is performed in two steps. First, whole blood is stimulated in the presence of specific antigens for 6â€"18 h, ending with cryopreservation of fixed white cells. These stimulation steps were specifically adapted to be practical and reliable in a rural, developing country field setting. Later, in a more resourceful setting, interferon-gamma producing CD4 + or CD8 + T cells are detected by flow cytometry. The assay proved sensitive and specific for detecting mycobacteria-specific immunity 10 weeks after Bacillus Calmette-Guerin (BCG) vaccination of newborns from a rural field site. © 2004 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Hanekom, Willem Albert
United States, Miami
University of Miami Leonard M. Miller School of Medicine
Hughes, Jane E.
South Africa, Cape Town
University of Cape Town
Mavinkurve, Maushumi
United States, New York
Rockefeller University
Mendillo, Megan
United States, Newark
Public Health Research Institute
Watkins, Marcia L.V.
South Africa, Cape Town
University of Cape Town
Gamieldien, Hoyam
South Africa, Cape Town
University of Cape Town
Gelderbloem, Sebastian J.
South Africa, Cape Town
University of Cape Town
Sidibana, Mzwandile
South Africa, Cape Town
University of Cape Town
Mansoor, Nazma
South Africa, Cape Town
University of Cape Town
Davids, Virginia
South Africa, Cape Town
University of Cape Town
Murray, Rose Ann
United States, Newark
Public Health Research Institute
Hawkridge, Anthony J.
South Africa, Cape Town
University of Cape Town
Haslett, Patrick A.J.
United States, Miami
University of Miami Leonard M. Miller School of Medicine
Ress, Stanley R.
South Africa, Cape Town
University of Cape Town
Hussey, Greg Dudley
South Africa, Cape Town
University of Cape Town
Kaplan, Gilla
United States, Newark
Public Health Research Institute
Statistics
Citations: 177
Authors: 16
Affiliations: 4
Identifiers
Doi:
10.1016/j.jim.2004.06.010
ISSN:
00221759