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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Exhaustive computational search of ionic-charge clusters that mediate interactions between mammalian cytochrome P450 (CYP) and P450-oxidoreductase (POR) proteins
Computational Biology and Chemistry, Volume 34, No. 1, Year 2010
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Description
In this work, a model for the interaction between CYP2B4 and the FMN domain of rat P450-oxidoreductase is built using as template the structure of a bacterial redox complex. Amino acid residues identified in the literature as cytochrome P450 (CYP)-redox partner interfacial residues map to the interface in our model. Our model supports the view that the bacterial template represents a specific electron transfer complex and moreover provides a structural framework for explaining previous experimental data. We have used our model in an exhaustive search for complementary pairs of mammalian CYP and P450-oxidoreductase (POR) charge clusters. We quantitatively show that among the previously defined basic clusters, the 433K-434R cluster is the most dominant (32.3% of interactions) and among the acidic clusters, the 207D-208D-209D cluster is the most dominant (29%). Our analysis also reveals the previously not described basic cluster 343R-345K (16.1% of interactions) and 373K (3.2%) and the acidic clusters 113D-115E-116E (25.8%), 92E-93E (12.9%), 101D (3.2%) and 179E (3.2%). Cluster pairings among the previously defined charge clusters include the pairing of cluster 421K-422R to cluster 207D-208D-209D. Moreover, 433K-434R and 207D-208D-209D, respectively the dominant positively and negatively charged clusters, are uncorrelated. Instead our analysis suggests that the newly identified cluster 113D-115E-116E is the main partner of the 433K-434R cluster while the newly described cluster 343R-345K is correlated to the cluster 207D-208D-209D. © 2009 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Zawaira, Alexander
South Africa, Observatory
Faculty of Health Sciences
Gallotta, Marco
South Africa, Cape Town
University of Cape Town
Beeton-Kempen, Natasha
South Africa, Observatory
Faculty of Health Sciences
Coulson, Lauren
South Africa, Observatory
Faculty of Health Sciences
Marais, Patrick C.
South Africa, Cape Town
University of Cape Town
Kuttel, Michelle M.
South Africa, Cape Town
University of Cape Town
Blackburn, Jonathan M.
South Africa, Observatory
Faculty of Health Sciences
Statistics
Citations: 7
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1016/j.compbiolchem.2009.10.006
ISSN:
14769271