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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java
Applied Microbiology and Biotechnology, Volume 103, No. 12, Year 2019
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Description
Discriminating between d-tartrate fermenting and non-fermenting strains of Salmonella enterica subsp. enterica serotype Paratyphi B is of major importance as these two variants have different pathogenic profiles. While d-tartrate non-fermenting S. Paratyphi B isolates are the causative agent of typhoid-like fever, d-tartrate fermenting isolates (also called variant Java) of the same serotype trigger the less dangerous gastroenteritis. The determination of S. Paratyphi B variants requires a time-consuming process and complex biochemical tests. Therefore, a quadruplex real-time PCR method, based on the allelic discrimination of molecular markers selected from the scientific literature and from whole genome sequencing data produced in-house, was developed in this study, to be applied to Salmonella isolates. This method was validated with the analysis of 178 S. Paratyphi B (d-tartrate fermenting and non-fermenting) and other serotypes reaching an accuracy, compared with the classical methods, of 98% for serotyping by slide agglutination and 100% for replacement of the biochemical test. The developed real-time PCR permits to save time and to obtain an accurate identification of a S. Paratyphi B serotype and its d-tartrate fermenting profile, which is needed in routine laboratories for fast and efficient diagnostics. © 2019, The Author(s).
Authors & Co-Authors
Mattheus, Wesley
Belgium, Brussels
Unit Bacterial Diseases Service
Saltykova, Assia
Belgium, Leuven
Interuniversity Microelectronics Centre
Belgium, Brussels
Sciensano
Roosens, Nancy H.C.
Belgium, Brussels
Sciensano
Dierick, Katelijne
Belgium, Brussels
Scientific Service Food-borne Pathogens
Marchal, Kathleen
Belgium, Leuven
Interuniversity Microelectronics Centre
Belgium, Ghent
Universiteit Gent
De Keersmaecker, Sigrid C.J.
Belgium, Brussels
Sciensano
Bertrand, Sophie
Belgium, Brussels
Sciensano
Statistics
Citations: 8
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1007/s00253-019-09854-4
ISSN:
01757598
Research Areas
Infectious Diseases