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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Detection of Ebola virus in oral fluid specimens during outbreaks of Ebola virus hemorrhagic fever in the Republic of Congo
Clinical Infectious Diseases, Volume 42, No. 11, Year 2006
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Description
Background. Patients who have refused to provide blood samples has meant that there have been significant delays in confirming outbreaks of Ebola virus hemorrhagic fever (EVHF). During the 2 EVHF outbreaks in the Republic of Congo in 2003, we assessed the use of oral fluid specimens versus serum samples for laboratory confirmation of cases of EVHF. Methods. Serum and oral fluid specimens were obtained from 24 patients with suspected Ebola and 10 healthy control subjects. Specimens were analyzed for immunoglobulin G antibodies by enzyme-linked immunosorbent assay (ELISA) and for Ebola virus by antigen detection ELISA and reverse-transcriptase polymerase chain reaction (RT-PCR). Oral fluid specimens were collected with a commercially available collection device. Results. We failed to detect antibodies against Ebola in the oral fluid specimens obtained from patients whose serum samples were seropositive. All patients with positive serum RT-PCR results also had positive results for their oral fluid specimens. Conclusions. This study demonstrates the usefulness of oral fluid samples for the investigation of Ebola outbreaks, but further development in antibodies and antigen detection in oral fluid specimens is needed before these samples are used for filovirus surveillance activities in Africa. © 2006 by the Infectious Diseases Society of America. All rights reserved.
Authors & Co-Authors
Formenty, Pierre Bernard Henri
Switzerland, Geneva
Organisation Mondiale de la Santé
Leroy, Éric Maurice
Gabon, Franceville
Centre International de Recherche Médicale de Franceville
Epelboin, Alain
France, Paris
Museum National D'histoire Naturelle
Libama, François
Switzerland, Geneva
Organisation Mondiale de la Santé
Lenzi, Marco
Switzerland, Geneva
Organisation Mondiale de la Santé
Sudeck, Hinrich
Germany, Hamburg
Bernhard Nocht Institut Fur Tropenmedizin Hamburg
Yaba, Philippe
Gabon, Franceville
Centre International de Recherche Médicale de Franceville
Allaranger, Yokouidé
Switzerland, Geneva
Organisation Mondiale de la Santé
Boumandlouki, Paul
Switzerland, Geneva
Organisation Mondiale de la Santé
Nkounkou, Virginot Blad
Congo
Ministère de la Santé et de la Population
Drosten, C.
Germany, Hamburg
Bernhard Nocht Institut Fur Tropenmedizin Hamburg
Grolla, Allen
Canada, Winnipeg
National Microbiology Laboratory
Feldmann, Heinz
Canada, Winnipeg
National Microbiology Laboratory
Roth, Cathy Ellen
Switzerland, Geneva
Organisation Mondiale de la Santé
Statistics
Citations: 141
Authors: 14
Affiliations: 6
Identifiers
Doi:
10.1086/503836
ISSN:
10584838
Research Areas
Health System And Policy
Infectious Diseases
Study Locations
Congo