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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Intercontinental emergence of Escherichia coli clone O25:H4-ST131 producing CTX-M-15
Journal of Antimicrobial Chemotherapy, Volume 61, No. 2, Year 2008
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Description
Background: Concomitant with the recent emergence of CTX-M-type extended-spectrum β-lactamases (ESBLs), Escherichia coli has become the enterobacterial species most affected by ESBLs. Multiple locales are encountering CTX-M-positive E. coli, including specifically CTX-M-15. To gain insights into the mechanism underlying this phenomenon, we assessed clonality and diversity of virulence profiles within an international collection of CTX-M-15-positive E. coli. Methods: Forty-one ESBL-positive E. coli isolates from eight countries and three continents (Europe, Asia and North America) were selected for study based on suspected clonality. Phylogenetic group, ERIC2 PCR profile, O H serotype, AmpC variant and antibiotic susceptibility were determined. Multilocus sequence typing (MLST) and PFGE provided additional discrimination. Virulence potential was inferred by detection of 46 virulence factor (VF) genes. Results: Thirty-six (88%) of the 41 E. coli isolates exhibited the same set of core characteristics: phylogenetic group B2, ERIC2 PCR profile 1, serotype O25:H4, AmpC EC6, ciprofloxacin resistance and MLST profile ST131. By PFGE, the 36 isolates constituted one large cluster at the 68% similarity level; this comprised 17 PFGE groups (defined at 85% similarity), some of which included strains from different countries. The 36 isolates exhibited highly (91% to 100%) similar VF profiles. Conclusions: We describe a broadly disseminated, CTX-M-15-positive and virulent E. coli clonal group with highly homogeneous virulence genotypes and subgroups exhibiting highly similar PFGE profiles, suggesting recent emergence. Understanding how this clone has emerged and successfully disseminated within the hospital and community, including across national boundaries, should be a public health priority. © The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
Authors & Co-Authors
Nicolas-Chanoine, Marie Hélène Sign©lène
France, Clichy
Hôpital Beaujon
France, Paris
Université Paris Cité
Leflon-Guibout, Véronique
France, Clichy
Hôpital Beaujon
Alonso, María Pilar
Unknown Affiliation
Caniça, Manuela M.
Portugal, Lisbon
Instituto Nacional de Saúde Dr. Ricardo Jorge
Park, Yeon-joon
South Korea, Bucheon-si
The Catholic University of Korea
Lavigne, Jean Philippe
France, Nimes
Centre Hospitalier Universitaire de Nîmes
Pitout, Johann D.D.
Canada, Calgary
Calgary Laboratory Services
Statistics
Citations: 692
Authors: 7
Affiliations: 8
Identifiers
Doi:
10.1093/jac/dkm464
ISSN:
14602091
Research Areas
Cancer
Health System And Policy