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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Gynogenesis induction and sex determination in the Eurasian perch, Perca fluviatilis
Aquaculture, Volume 243, No. 1-4, Year 2005
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Description
In the present study, we used meiotic gynogenesis, widely used in studies on sex determination, to confirm female homogamety in Eurasian perch, Perca fluviatilis. Sperm irradiated with UV for 400 s was used to artificially fertilized eggs. The diploid of the resulting embryos was restored by a heat shock (30°C) applied to the eggs 5 min postfertilization, for 25 min. Fertilization (ranging between 45% and 75%) and survival rates at hatching (ranging between 3.4% and 46.6%) were not significantly different (P>0.05) between the diploid control and gynogenetics. The diploid controls and two batches of gynogenetics contained 100% diploid larvae, whereas two other batches of gynogenetics contained 6.7% and 10.0% triploid larvae. The sex ratios of the diploid controls were not significantly different from 1:1, whereas all gynogenetic families were 100% female. These results confirm female homogamety in Eurasian perch, demonstrated by the use of hormonally mascilinized breeders in a previous study. © 2004 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Rougeot, Carole
Belgium, Liege
Université de Liège
Ngingo, Jean Virimumbalu
Congo, Butembo
Université Catholique du Graben
Gillet, Laurent
Belgium, Liege
Université de Liège
Vanderplasschen, Alain F.C.
Belgium, Liege
Université de Liège
Mélard, Charles
Belgium, Liege
Université de Liège
Statistics
Citations: 22
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1016/j.aquaculture.2004.11.004
ISSN:
00448486
Participants Gender
Female