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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Isolation and characterization of new Leptospira genotypes from patients in Mayotte (Indian Ocean)
PLoS Neglected Tropical Diseases, Volume 4, No. 6, Article e724, Year 2010
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Description
Background: Leptospirosis has been implicated as a severe and fatal form of disease in Mayotte, a French-administrated territory located in the Comoros archipelago (southwestern Indian Ocean). To date, Leptospira isolates have never been isolated in this endemic region. Methods and Findings: Leptospires were isolated from blood samples from 22 patients with febrile illness during a 17- month period after a PCR-based screening test was positive. Strains were typed using hyper-immune antisera raised against the major Leptospira serogroups: 20 of 22 clinical isolates were assigned to serogroup Mini; the other two strains belonged to serogroups Grippotyphosa and Pyrogenes, respectively. These isolates were further characterized using partial sequencing of 16S rRNA and ligB gene, Multi Locus VNTR Analysis (MLVA), and pulsed field gel electrophoresis (PFGE). Of the 22 isolates, 14 were L. borgpetersenii strains, 7 L. kirschneri strains, and 1, belonging to serogoup Pyrogenes, was L. interrogans. Results of the genotyping methods were consistent. MLVA defined five genotypes, whereas PFGE allowed the recognition of additional subgroups within the genotypes. PFGE fingerprint patterns of clinical strains did not match any of the patterns in the reference strains belonging to the same serogroup, suggesting that the strains were novel serovars. Conclusions: Preliminary PCR screening of blood specimen allowed a high isolation frequency of leptospires among patients with febrile illness. Typing of leptospiral isolates showed that causative agents of leptospirosis in Mayotte have unique molecular features. © 2010 Bourhy et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2889827/bin/pntd.0000724.s001.doc
Authors & Co-Authors
Bourhy, Pascale
France, Paris
Institut Pasteur, Paris
Collet, Louis
Mayotte, Mamoudzou
Centre Hospitalier de Mayotte
Clément, Sabine
France, Paris
Institut Pasteur, Paris
Huerre, Michel René
France, Paris
Institut Pasteur, Paris
Avé, Patrick
France, Paris
Institut Pasteur, Paris
Giry, Claude
Mayotte, Mamoudzou
Centre Hospitalier de Mayotte
Pettinelli, François
Mayotte, Mamoudzou
Centre Hospitalier de Mayotte
Picardeau, Mathieu
France, Paris
Institut Pasteur, Paris
Statistics
Citations: 73
Authors: 8
Affiliations: 2
Identifiers
Doi:
10.1371/journal.pntd.0000724
Research Areas
Genetics And Genomics
Study Locations
Comoros