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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Multiplex PCR for direct identification of Campylobacter spp. in human and chicken stools
Journal of Medical Microbiology, Volume 56, No. 10, Year 2007
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Description
Differentiation between Campylobacter jejuni and Campylobacter coli is problematic in clinical specimens due to fastidious growth requirements and limited biochemical tests. This study describes a rapid, multiplex PCR protocol for the direct detection and differentiation of C. jejuni and C. coli in stools. An evaluation was carried out of this multiplex protocol based on the detection of cadF (genus specific), and hipO (C. jejuni) and asp (C. coli) genes, using stool from patients with Campylobacter enteritis and chicken. Protocol sensitivity was assessed and specificity determined using a panel of enteric bacteria, and evaluation of 30 diarrhoeic stool specimens culture negative for Campylobacter. Of the 114 specimens (54 human and 60 chicken) evaluated by the protocol, 70 (61.4 %) were identified as C. jejuni, 35 (30.7 %) as C. coli and 9 (7.9 %) as a mixed infection/colonization with both species. All mixed infections were identified as C. jejuni by culture. Among the stool specimens that were culture negative for Campylobacter, two (6.7%) were C. jejuni positive by multiplex PCR. The protocol sensitivity limit was 0.015-0.016 ng C. jejuni and C. coli DNA μl-1 in the specimen. There was no cross-reaction with the reference strains assessed. Comparison of hippurate test and multiplex PCR demonstrated 17 isolates with false-positive hippurate enzymic activity and 7 with false-negative activity. This rapid protocol (turnaround time 6 h) is highly sensitive and specific for direct evaluation of stool for these pathogens. It has significant application for routine clinical diagnostic and epidemiological purposes. © 2007 SGM.
Authors & Co-Authors
Al Amri, Aisha
Bahrain, Manama
Arabian Gulf University
Senok, Abiola C.
United Arab Emirates, Sharjah
College of Medicine
Ismaeel, Abdulrahman Yusuf
Bahrain, Manama
Arabian Gulf University
Al-Mahmeed, Ali E.
Bahrain, Manama
Arabian Gulf University
Botta, Giuseppe A.
Bahrain, Manama
Arabian Gulf University
Italy, Udine
Università Degli Studi Di Udine, Dipartimento Di Area Medica
Statistics
Citations: 95
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1099/jmm.0.47220-0
ISSN:
00222615
Research Areas
Genetics And Genomics