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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
An aptamer that neutralizes R5 strains of human immunodeficiency virus type 1 blocks gp120-CCR5 interaction
Journal of Virology, Volume 79, No. 21, Year 2005
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Description
We recently described the isolation and structural characterization of 2′-fluoropyrimidine-substitiited RNA aptamers that bind to gp120 of R5 strains of human immunodeficiency virus type 1 and thereby potently neutralize the infectivity of phylogenetically diverse R5 strains. Here we investigate the physical basis of their antiviral action. We show that both N-linked oligosaccharides and the variable loops V1/V2 and V3 are not required for binding of one aptamer, B40, to gp120. Using surface plasmon resonance binding analyses, we show that the aptamer binds to the CCR5-binding site on gp120 in a relatively CD4-independent manner, providing a mechanistic explanation for its neutralizing potency. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Dey, Antu K.
United Kingdom, Oxford
University of Oxford Medical Sciences Division
United Kingdom, Oxford
Sir William Dunn School of Pathology
United States, New York
Weill Cornell Medicine
Khati, Makobetsa
United Kingdom, Oxford
Sir William Dunn School of Pathology
South Africa, Cape Town
Faculty of Health Sciences
Tang, Min
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Wyatt, Richard
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Lea, Susan Mary
United Kingdom, Oxford
University of Oxford Medical Sciences Division
James, William S.
United Kingdom, Oxford
Sir William Dunn School of Pathology
Statistics
Citations: 83
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1128/JVI.79.21.13806-13810.2005
ISSN:
0022538X