Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
CXCL10/IP-10 release is induced by incubation of whole blood from tuberculosis patients with ESAT-6, CFP10 and TB7.7
Microbes and Infection, Volume 9, No. 7, Year 2007
Notification
URL copied to clipboard!
Description
IFN-γ responses to Mycobacterium tuberculosis specific antigens are used as in vitro diagnostic tests for tuberculosis infection. The tests are sensitive and specific for latent and active tuberculosis disease, but sensitivity may be reduced during immunosuppression. The objective of the study was to explore new ways to improve the diagnosis of tuberculosis infection using CXCL10 and IL-2 as alternative markers to IFN-γ. CXCL10, IL-2, and IFN-γ responses to stimulation with ESAT-6/CFP10/TB7.7 were assessed in 12 Quantiferon positive, 8 Quantiferon negative tuberculosis patients and 11 Quantiferon negative controls. CXCL10 and IL-2 were determined by multiplex and IFN-γ by the Quantiferon ELISA. The median antigen specific CXCL10, IFN-γ, and IL-2 responses in patients with tuberculosis were 870 pg/ml (range 261-1576 pg/ml), 217 pg/ml (81-1273 pg/ml), 59 pg/ml (14-276 pg/ml) respectively, and the CXCL10 responses were significantly higher than any of the other cytokines measured (p = 0.001). In 4/7 individuals with a negative (n = 6) or indeterminate (n = 1) Quantiferon test, antigen specific CXCL10 responses were detectable at high levels ranging from 196-532 pg/ml. In conclusion CXCL10 was strongly induced after M. tuberculosis specific stimulation and sensitivity appeared superior to the Quantiferon test. Our findings suggest that CXCL10 may serve as an alternative or additional marker for the immunodiagnosis of tuberculosis. © 2007 Elsevier Masson SAS. All rights reserved.
Authors & Co-Authors
Rühwald, Morten
Denmark, Copenhagen
Copenhagen University Hospital
Bjerregaard-Andersen, Morten
Denmark, Aarhus
Aarhus Universitetshospital
Guinea-bissau, Bissau
Bandim Health Project
Rabna, Paulo
Guinea-bissau, Bissau
Bandim Health Project
Kofoed, K.
Denmark, Copenhagen
Copenhagen University Hospital
Eugen-Olsen, Jesper
Denmark, Copenhagen
Copenhagen University Hospital
Ravn, Pernille
Denmark, Copenhagen
Copenhagen University Hospital
Statistics
Citations: 113
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/j.micinf.2007.02.021
ISSN:
12864579
e-ISSN:
1769714X