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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Increased DNA damage in children caused by passive smoking as assessed by comet assay and oxidative stress
Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Volume 629, No. 2, Year 2007
Notification
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Description
The present study aimed to evaluate the association between the environmental tobacco smoke (ETS) and DNA damage in relation to oxidative stress (OS) in children. Sixty-four children of age 1-8 years, selected from the outpatient clinic of Mansoura University Children Hospital were divided into two groups (23 children/group) based on high (>20 cigarettes/day) or low (<20 cigarettes/day) exposure to ETS at home. Twenty symptom-free children with normal cotinine level and with no exposure to ETS were recruited as controls. The comet assay was used to quantify the level of DNA damage in lymphocytes isolated from all children. Spectrophotometric methods were used to assess the serum level of malondialdehyde (MDA) and activity of glutathione peroxidase (GSH-Px) in erythrocytes. Also, serum level of tocopherol fractions (α, γ, δ) was assessed by high performance liquid chromatography (HPLC). Children exposed to ETS exhibited retarded growth, more chest problems, and gastroenteritis than the control. A significant increase in mean comet tail length indicating DNA damage was observed in ETS-exposed children (P < 0.001) compared to controls. ETS-exposed children had significantly (P < 0.001) higher MDA level paralleled with significant (P < 0.001) decrease in the level of GSH-Px and tocopherol fractions compared with controls. The GSH-Px activity and tocopherol levels were inversely correlated with the increase of ETS exposure. These results show that inhalation of ETS is associated with an increase in the level of oxidants and a simultaneous decrease in the level of antioxidants in the children's blood. This status of oxidant-antioxidant imbalance (OS) may be one of the mechanisms leading to DNA damage detected in lymphocytes of ETS-exposed children. In conclusion, the present study gives an indication of an association between DNA damage and ETS exposure in children. © 2007 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Zalata, Adel Abd El Kader
Egypt, Mansoura
Faculty of Medicine
Yahia, Sohier
Egypt, Mansoura
Faculty of Medicine
El-Bakary, Amal A.
Egypt, Mansoura
Faculty of Medicine
Elsheikha, Hany M.
United Kingdom, Nottingham
University of Nottingham, Faculty of Medicine and Health Sciences
Statistics
Citations: 43
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1016/j.mrgentox.2007.02.001
ISSN:
13835718
Research Areas
Genetics And Genomics
Health System And Policy
Maternal And Child Health
Substance Abuse