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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Comprehensive profiles of human milk oligosaccharides yield highly sensitive and specific markers for determining secretor status in lactating mothers
Journal of Proteome Research, Volume 11, No. 12, Year 2012
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Description
Human milk oligosaccharides (HMOs), as an abundant and bioactive component of breast milk, work in many ways to promote the health of breast fed infants. The expression of HMOs has been shown to vary in accordance with Lewis blood type and secretor status, as women of different blood types differ in the expression of α1,2 fucosyltransferase (FUT2) and α1,3/4 fucosyltransferase (FUT3). In this study, HMOs were extracted from the milk of 60 women from The Gambia, Africa with various Lewis and secretor blood types. The HMOs were profiled using high resolution HPLC-Chip/TOF mass spectrometry. Notably, the amounts of fucosylation varied significantly between Le(a+b-) nonsecretors, Le(a-b+) and Le(a-b-) secretors, and Le(a-b-) nonsecretors. With higher frequency of expression of the recessive Lewis negative and nonsecretor phenotypes in West African populations, the HMO profiles of several milks from women of these phenotypes were examined, demonstrating decreased amounts of total oligosaccharide abundance and lower relative amounts of fucosylation. Also in this study, four specific fucosylated structures (2'FL, LNFP I, LDFT, and LNDFH I) were determined to be specific and sensitive glycan markers for rapidly determining secretor status without the need for serological testing. © 2012 American Chemical Society.
Authors & Co-Authors
Totten, Sarah M.
United States, Davis
University of California, Davis
Zivkovic, Angela M.
United States, Davis
University of California, Davis
Darboe, Momodou K.
Gambia, Banjul
Medical Research Council Laboratories Gambia
German, Bruce Bruce
United States, Davis
University of California, Davis
Prentice, Andrew M.
Gambia, Banjul
Medical Research Council Laboratories Gambia
Lebrilla, Carlito B.
United States, Davis
University of California, Davis
Statistics
Citations: 166
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1021/pr300769g
ISSN:
15353907
Research Areas
Maternal And Child Health
Study Locations
Gambia
Participants Gender
Female