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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Identification of in vivo-induced bacterial protein antigens during human infection with Salmonella enterica serovar Typhi
Infection and Immunity, Volume 74, No. 9, Year 2006
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Description
We applied an immunoscreening technique, in vivo-induced antigen technology (IVIAT), to identify immunogenic bacterial proteins expressed during human infection with Salmonella enterica serovar Typhi, the cause of typhoid fever. We were able to assign a functional classification to 25 of 35 proteins identified by IVIAT. Of these 25, the majority represent proteins with known or potential roles in the pathogenesis of S. enterica. These include proteins implicated in fimbrial structure and biogenesis, antimicrobial resistance, heavy metal transport, bacterial adhesion, and extracytoplasmic substrate trafficking as well as secreted hydrolases. The 10 remaining antigens represent proteins with unknown functions. Of the 35 identified antigens, four had no immunoreactivity when probed with control sera from individuals never exposed to serovar Typhi organisms; these four included PagC, TcfB, and two antigens of unknown function encoded by STY0860 and STY3683. PagC is a virulence factor known to be upregulated in vivo in S. enterica serovar Typhimurium infection of mice. TcfB is the major structural subunit of a fimbrial operon found in serovar Typhi with no homolog in serovar Typhimurium organisms. By examining differential immunoreactivities in acute- versus convalescent-phase human serum samples, we found specific anti-PagC and anti-TcfB immunoglobulin G responses in patients with serovar Typhi bacteremia. Serovar Typhi antigens identified by IVIAT warrant further evaluation for their contributions to pathogenesis, and they may have diagnostic, therapeutic, or preventive uses. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Harris, Jason B.
United States, Boston
Massachusetts General Hospital
United States, Boston
Harvard Medical School
LaRocque, Regina C.
United States, Boston
Massachusetts General Hospital
United States, Boston
Harvard Medical School
Qadri, Firdausi
Bangladesh, Dhaka
International Centre for Diarrhoeal Disease Research Bangladesh
Calderwood, Stephen Beaven
United States, Boston
Massachusetts General Hospital
United States, Boston
Harvard Medical School
Hohmann, Elizabeth L.
United States, Boston
Massachusetts General Hospital
United States, Boston
Harvard Medical School
Breiman, Robert F.
Bangladesh, Dhaka
International Centre for Diarrhoeal Disease Research Bangladesh
Brooks, W. Abdullah
Bangladesh, Dhaka
International Centre for Diarrhoeal Disease Research Bangladesh
Ryan, Edward Thomas
United States, Boston
Massachusetts General Hospital
United States, Boston
Harvard Medical School
United States, Boston
Harvard T.h. Chan School of Public Health
Statistics
Citations: 61
Authors: 8
Affiliations: 5
Identifiers
Doi:
10.1128/IAI.00488-06
ISSN:
00199567
Research Areas
Infectious Diseases