Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Multiple bud cultures of 'Barhee' date palm (Phoenix dactylifera) and physiological status of regenerated plants
Journal of Plant Physiology, Volume 168, No. 14, Year 2011
Notification
URL copied to clipboard!
Description
Adventitious bud clusters of date palm 'Barhee' were successfully established from juvenile leaves (<1cm) using reduced amounts of 2,4-D (0.2mgL-1) to limit the risk of somaclonal variation. An average of 8.4 adventitious buds per explant were obtained. Histological examination showed that the superficial cell layers of leaves had the highest caulogenic capacity. High sucrose concentration (70gL-1) was used for the conversion of initial buds to multiple bud clusters. The promoting effect of temporary immersion on shoot proliferation was found to be significant when compared to cultivation on solid media. Elongation of shoots was also better using a thin film of PGR-free liquid medium instead of a solid medium. Anatomical observations indicated that roots from vitroplants were potentially functional at various developmental stages. However, only 12-month-old vitroplants were found to be physiologically able to control transpirational vapor loss. Additionally, the photochemical activity of photosystem II in these vitroplants was close to that measured in plants that were already acclimatized. As a result, 83.3% of regenerated plants were successfully acclimatized. No phenotypic variation was observed among more than 500 adventitious bud-derived plants. All regenerants survived after field transplantation. We found that the production of adventitious bud clusters in small bioreactors was able to provide an efficient micropropagation system for date palm cv. 'Barhee'. An in vitro hardening step was a prerequisite for the successful transfer of vitroplants in soil. © 2011 Elsevier GmbH.
Authors & Co-Authors
Fki, Lotfi
Tunisia, Sfax
Faculté Des Sciences de Sfax
Bouaziz, N.
Tunisia, Sfax
Faculté Des Sciences de Sfax
Kriaâ, Walid
Tunisia, Sfax
Faculté Des Sciences de Sfax
Benjemaa-Masmoudi, Raja
Tunisia, Sfax
Faculté Des Sciences de Sfax
Gargouri-Bouzid, Radhia
Tunisia, Sfax
University of Sfax
Rival, Alain
France, Montpellier
Diversité, Adaptation et Développement Des Plantes
Drira, Noureddine
Tunisia, Sfax
Faculté Des Sciences de Sfax
Statistics
Citations: 46
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1016/j.jplph.2011.03.013
ISSN:
01761617