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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Apoptosis-associated speck-like protein (ASC) controls Legionella pneumophila infection in human monocytes
Journal of Biological Chemistry, Volume 286, No. 5, Year 2011
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Description
The ability of Legionella pneumophila to cause pneumonia is determined by its capability to evade the immune system and grow within human monocytes and their derived macrophages. Human monocytes efficiently activate caspase-1 in response to Salmonella but not to L. pneumophila. The molecular mechanism for the lack of inflammasome activation during L. pneumophila infection is unknown. Evaluation of the expression of several inflammasome components in human monocytes during L. pneumophila infection revealed that the expression of the apoptosis-associated speck-like protein (ASC) and the NOD-like receptor NLRC4 are significantly down-regulated in human monocytes. Exogenous expression of ASC maintained the protein level constant during L. pneumophila infection and conveyed caspase-1 activation and restricted the growth of the pathogen. Further depletion of ASC with siRNA was accompanied with improved NF-κB activation and enhanced L. pneumophila growth. Therefore, our data demonstrate that L. pneumophila manipulates ASC levels to evade inflammasome activation and grow in human monocytes. By targeting ASC, L. pneumophila modulates the inflammasome, the apoptosome, and NF-κB pathway simultaneously. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3030324/bin/supp_286_5_3203__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3030324/bin/supp_M110.197681_jbc.M110.197681-3.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3030324/bin/supp_M110.197681_jbc.M110.197681-4.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3030324/bin/supp_M110.197681_jbc.M110.197681-2.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3030324/bin/supp_M110.197681_Suppl_figure_1.pdf
Authors & Co-Authors
Abdelaziz, Dalia H.
United States, Columbus
The Ohio State University
Egypt, Helwan
Faculty of Pharmacy
Gavrilin, Mikhail A.
United States, Columbus
The Ohio State University
Akhter, Anwari
United States, Columbus
The Ohio State University
Caution, Kyle
United States, Columbus
The Ohio State University
Kotrange, Sheetal
United States, Columbus
The Ohio State University
Abu Khweek, Arwa
United States, Columbus
The Ohio State University
Abdulrahman, Basant A.
United States, Columbus
The Ohio State University
Egypt, Helwan
Faculty of Pharmacy
Grandhi, Jaykumar
United States, Columbus
The Ohio State University
Hassan, Zeinab A.
Egypt, Helwan
Faculty of Pharmacy
Marsh, Clay
United States, Columbus
The Ohio State University
Wewers, Mark D.
United States, Columbus
The Ohio State University
Amer, Amal O.
United States, Columbus
The Ohio State University
Statistics
Citations: 64
Authors: 12
Affiliations: 2
Identifiers
Doi:
10.1074/jbc.M110.197681
ISSN:
00219258
e-ISSN:
1083351X
Research Areas
Cancer
Infectious Diseases