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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
MALDI imaging mass spectrometry in ovarian cancer for tracking, identifying, and validating biomarkers
Medical Science Monitor, Volume 16, No. 8, Year 2010
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Description
Background: Among biomarkers, cancer-antigen 125 (CA-125) is the most studied. We propose an analytical tool to track ovarian carcinoma biomarkers, that is, the MALDI mass spectrometry imaging. Material/Methods: Ovarian carcinomas and benign ovaries were directly analyzed by MALDI-TOF-MS. After automatic profiling and mass spectrometry imaging analyses, hierarchical clustering based on principal component analysis in nonsupervised mode was carried out. On the same samples, preparations were performed to investigate peptides, then proteins, followed by high mass proteins, in an automatic profiling to specific signatures for diagnosis. Using tissue bottom-up strategy on tissue digestion, and mass spectrometry imaging after by shotgun sequencing by nalano-LC-IT-MS in MS/MS mode from washing samples from on tissue digested peptides, several biomarkers were found. Results: A list of specific biomarkers from the ovarian carcinoma regions was obtained and classified as proteins associated with cell proliferation, involved in immune response modulation, signaling to the cytoskeleton, and tumor progression. These specific biomarkers were then validated by immunocytochemistry using Tag-mass technology, cell biology, Western blot, and by PCR (using SKOV-3 ovarian epithelial cancer cells). A link between the immune regulation (innate immunity, tolerance) and virus cause is also discussed. Conclusions: From the biomarkers identified, proteins involved in immune response modulation and cell proliferation have been pointed out in this study. Two new markers have been identified using such a strategy, that is, fragment C-terminal of the PSME1 (Reg-Alpha) and mucin-9. © Med Sci Monit, 2010.
Authors & Co-Authors
Ayed, Mohamed El
France, Lille
Université de Lille
Tunisia, Monastir
Faculté de Médecine de Monastir
Bonnel, David
France, Lille
Université de Lille
Canada, Sherbrooke
Institut de Pharmacologie de Sherbrooke
Longuespée, Remi
France, Lille
Université de Lille
Canada, Sherbrooke
Institut de Pharmacologie de Sherbrooke
Castellier, Céline
France, Lille
Université de Lille
France, Lille
Chu Lille
Franck, Julien
France, Lille
Université de Lille
Vergara, Daniele
France, Lille
Université de Lille
Desmons, Annie
France, Lille
Université de Lille
Tasiemski, Aurélie A.
France, Lille
Université de Lille
Kénani, Abderraouf
Tunisia, Monastir
Faculté de Médecine de Monastir
Vinatier, D.
France, Lille
Chu Lille
Day, Robert
Canada, Sherbrooke
Institut de Pharmacologie de Sherbrooke
Fournier, Isabelle
France, Lille
Université de Lille
SALZET, Michel
France, Lille
Université de Lille
Statistics
Citations: 13
Authors: 13
Affiliations: 4
Research Areas
Cancer