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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
CD26/dipeptidyl peptidase IV (CD26/DPPIV) is highly expressed in peripheral blood of HIV-1 exposed uninfected Female sex workers
Virology Journal, Volume 7, Article 343, Year 2010
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Description
Background. Design of effective vaccines against the human immunodeficiency virus (HIV-1) continues to present formidable challenges. However, individuals who are exposed HIV-1 but do not get infected may reveal correlates of protection that may inform on effective vaccine design. A preliminary gene expression analysis of HIV resistant female sex workers (HIV-R) suggested a high expression CD26/DPPIV gene. Previous studies have indicated an anti-HIV effect of high CD26/DPPIV expressing cells in vitro. Similarly, high CD26/DPPIV protein levels in vivo have been shown to be a risk factor for type 2 diabetes. We carried out a study to confirm if the high CD26/DPPIV gene expression among the HIV-R were concordant with high blood protein levels and its correlation with clinical type 2 diabetes and other perturbations in the insulin signaling pathway. Results. A quantitative CD26/DPPIV plasma analysis from 100 HIV-R, 100 HIV infected (HIV +) and 100 HIV negative controls (HIV Neg) showed a significantly elevated CD26/DPPIV concentration among the HIV-R group (mean 1315 ng/ml) than the HIV Neg (910 ng/ml) and HIV + (870 ng/ml, p < 0.001). Similarly a FACs analysis of cell associated DPPIV (CD26) revealed a higher CD26/DPPIV expression on CD4+ T-cells derived from HIV-R than from the HIV+ (90.30% vs 80.90 p = 0.002) and HIV Neg controls (90.30% vs 82.30 p < 0.001) respectively. A further comparison of the mean fluorescent intensity (MFI) of CD26/DPPIV expression showed a higher DPP4 MFI on HIV-R CD4+ T cells (median 118 vs 91 for HIV-Neg, p = 0.0003). An evaluation for hyperglycemia, did not confirm Type 2 diabetes but an impaired fasting glucose condition (5.775 mmol/L). A follow-up quantitative PCR analysis of the insulin signaling pathway genes showed a down expression of NFB, a central mediator of the immune response and activator of HIV-1 transcription. Conclusion. HIV resistant sex workers have a high expression of CD26/DPPIV in tandem with lowered immune activation markers. This may suggest a novel role for CD26/DPPIV in protection against HIV infection in vivo. © 2010 Songok et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Songok, Elijah Maritim
Kenya, Nairobi
Virus Research Centre
Canada, Winnipeg
University of Manitoba
Kenya, Nairobi
University of Nairobi College of Health Sciences
Osero, Bernard
Kenya, Nairobi
Virus Research Centre
McKinnon, Lyle R.
Kenya, Nairobi
University of Nairobi College of Health Sciences
Canada, Toronto
University of Toronto
Rono, Martin Kibet
Kenya, Nairobi
Virus Research Centre
Apidi, Winnie
Canada, Winnipeg
University of Manitoba
Kenya, Nairobi
University of Nairobi College of Health Sciences
Matey, E.
Kenya, Nairobi
Virus Research Centre
Meyers, Adrienne F.A.
Canada, Winnipeg
University of Manitoba
Kenya, Nairobi
University of Nairobi College of Health Sciences
Canada, Ottawa
Public Health Agency of Canada
Luo, Ma
Canada, Winnipeg
University of Manitoba
Kenya, Nairobi
University of Nairobi College of Health Sciences
Canada, Ottawa
Public Health Agency of Canada
Kimani, Joshua
Canada, Winnipeg
University of Manitoba
Kenya, Nairobi
University of Nairobi College of Health Sciences
Wachihi, Charles
Kenya, Nairobi
University of Nairobi College of Health Sciences
Ball, Terry Blake
Canada, Winnipeg
University of Manitoba
Kenya, Nairobi
University of Nairobi College of Health Sciences
Canada, Ottawa
Public Health Agency of Canada
Plummer, Francis Allan
Canada, Winnipeg
University of Manitoba
Canada, Ottawa
Public Health Agency of Canada
Mpoke, Solomon S.
Kenya, Nairobi
Virus Research Centre
Statistics
Citations: 18
Authors: 13
Affiliations: 5
Identifiers
Doi:
10.1186/1743-422X-7-343
e-ISSN:
1743422X
Research Areas
Genetics And Genomics
Infectious Diseases
Noncommunicable Diseases
Study Design
Cohort Study
Study Approach
Quantitative
Participants Gender
Female