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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Isolation and characterization of human monoclonal antibodies from individuals infected with West Nile Virus
Journal of Virology, Volume 80, No. 14, Year 2006
Notification
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Description
Monoclonal antibodies (MAbs) neutralizing West Nile Virus (WNV) have been shown to protect against infection in animal models and have been identified as a correlate of protection in WNV vaccine studies. In the present study, antibody repertoires from three convalescent WNV-infected patients were cloned into an scFv phage library, and 138 human MAbs binding to WNV were identified. One hundred twenty-one MAbs specifically bound to the viral envelope (E) protein and four MAbs to the premembrane (prM) protein. Enzyme-linked immunosorbent assay-based competitive-binding assays with representative E protein-specific MAbs demonstrated that 24/51 (47%) bound to domain II while only 4/51 (8%) targeted domain III. In vitro neutralizing activity was demonstrated for 12 MAbs, and two of these, CR4374 and CR4353, protected mice from lethal WNV challenge at 50% protective doses of 12.9 and 357 μg/kg of body weight, respectively. Our data analyzing three infected individuals suggest that the human anti-WNV repertoire after natural infection is dominated by nonneutralizing or weakly neutralizing MAbs binding to domain II of the E protein, while domain III-binding MAbs able to potently neutralize WNV in vitro and in vivo are rare. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC1489037/bin/jvirol_80_14_6982__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC1489037/bin/jvirol_80_14_6982__Suppl_Table_1.doc
Authors & Co-Authors
Throsby, Mark
Netherlands, Leiden
Crucell nv
Geuijen, Cecile
Netherlands, Leiden
Crucell nv
Goudsmit, Jaap
Netherlands, Leiden
Crucell nv
Bakker, Arjen Q.
Netherlands, Leiden
Crucell nv
Korimbocus, Jehanara
France, Paris
Institut Pasteur, Paris
Kramer, R. Arjen
Netherlands, Leiden
Crucell nv
Clijsters-Van Der Horst, Marieke
Netherlands, Leiden
Crucell nv
De Jong, Maureen
Netherlands, Leiden
Crucell nv
Jongeneelen, Mandy A.C.
Netherlands, Leiden
Crucell nv
Thijsse, Sandra
Netherlands, Leiden
Crucell nv
Smit, Renate
Netherlands, Leiden
Crucell nv
Visser, Therese J.
Netherlands, Leiden
Crucell nv
Bijl, Nora
Netherlands, Leiden
Crucell nv
Netherlands, Amsterdam
Amsterdam Umc - University of Amsterdam
Marissen, Wilfred E.
Netherlands, Leiden
Crucell nv
Loeb, Mark B.
Canada, Hamilton
Mcmaster University
Kelvin, David J.
Canada, Toronto
University Health Network University of Toronto
Preiser, Wolfgang
Germany, Frankfurt am Main
Goethe-universität Frankfurt am Main
South Africa, Stellenbosch
Stellenbosch University
Ter Meulen, Jan H.
Netherlands, Leiden
Crucell nv
de Kruif, John
Netherlands, Leiden
Crucell nv
Statistics
Citations: 173
Authors: 19
Affiliations: 7
Identifiers
Doi:
10.1128/JVI.00551-06
ISSN:
0022538X