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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Sleeping sickness diagnosis: Use of buffy coats improves the sensitivity of the mini anion exchange centrifugation test
Tropical Medicine and International Health, Volume 15, No. 7, Year 2010
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Description
Objectives: To evaluate a modification of the mini anion exchange centrifugation test (mAECT) for the diagnosis of Trypanosoma brucei (T.b.) gambiense human African trypanosomiasis (HAT). To increase its sensitivity, this test uses 350 ml of buffy coat withdrawn from 5 ml of blood instead of blood. Methods: The new protocol was first tested experimentally on serial dilution of trypanosomes and was then further evaluated under field conditions on 57 patients with HAT diagnosed during a medical survey in Guinea. Results: Experimentally, the use of buffy coats improved mAECT sensitivity at least five fold and enabled to consistently detect parasites in blood at a concentration of 10 trypanosomesl;ml. During the field evaluation, more patients tested positive by mAECT-bc (96.5%) than by mAECT-blood (78.9%, chi;2 = 6.93, P = 0.008) and lymph juice examination (77.2%, chi;2 = 7.67, P = 0.005). Furthermore, the number of parasites per collectors was significantly higher (7.2 vs. 2.6, P = 0.001) when buffy coats were used instead of blood. Conclusion: The use of the mAECT-bc protocol enabled a significant improvement of HAT parasitological diagnosis in Guinea, without any additional costs. It would deserve to be tested in other T.b. gambiense endemic areas. © 2010 Blackwell Publishing Ltd.
Authors & Co-Authors
Camara, Mamadou I.
Guinea
Programme National de Lutte Contre la Trypanosomose Humaine Africaine
Camara, Oumou B.
Guinea
Programme National de Lutte Contre la Trypanosomose Humaine Africaine
Burkina Faso
Centre International de Recherche-développement Sur L'elevage en Zone Subhumide
Ilboudo, Hamidou
Burkina Faso
Centre International de Recherche-développement Sur L'elevage en Zone Subhumide
Sakandé, Hassane
Burkina Faso
Centre International de Recherche-développement Sur L'elevage en Zone Subhumide
Kaboré, Jacques
Burkina Faso
Centre International de Recherche-développement Sur L'elevage en Zone Subhumide
N'Dri, Louis
Cote D'ivoire, Bouake
Institut Pierre Richet Bouake
Jamonneau, Vincent
Burkina Faso
Centre International de Recherche-développement Sur L'elevage en Zone Subhumide
France, Montpellier
Ird Centre de Montpellier
Bucheton, Bruno
Burkina Faso
Centre International de Recherche-développement Sur L'elevage en Zone Subhumide
France, Montpellier
Ird Centre de Montpellier
Statistics
Citations: 67
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1111/j.1365-3156.2010.02546.x
ISSN:
13602276
e-ISSN:
13653156
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Guinea