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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Identification of a bovine surface antigen uniquely expressed on CD4
−
CD8
−
T cell receptor γ/δ
+
T lymphocytes
European Journal of Immunology, Volume 20, No. 4, Year 1990
Notification
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Description
In this study, two monoclonal antibodies, IL‐A29 and CC15, are described that identify a novel bovine cell surface marker of 215/300 kDa. The antibodies reacted with a discrete population of resting lymphocytes in peripheral blood which, in young animals, constituted about 25% of the mononuclear cells. Thymus, lymph nodes and spleen contained < 5% positive cells. These cells were negative for surface Ig, a monocyte/granulocyte marker, and the T lymphocyte antigens CD2, CD6, CD4 and CD8. Immunohistological analyses revealed the presence of IL‐A29/CC15‐positive lymphocytes in the thymic medulla, in the outer cortex of lymph nodes, in the marginal zones of the spleen, in the dermal and epidermal layers of the skin and in the lamina propria of the gut. The IL‐A29/CC15+ cells in unfractionated blood mononuclear cells responded in autologous and allogeneic mixed lymphocyte cultures, and when purified they responded to concanavalin A in the presence of recombinant interleukin 2. These observations suggested this population of cells belonged to the T cell lineage. In order to unambiguously define their lineage, cDNA clones encoding bovine T cell receptor (TcR) and CD3 proteins were isolated. Northern blot analyses of IL‐A29/CC15+ cell populations and of established cell lines of various lineages demonstrated that they expressed TcR δ and CD3γ, δ and ϵ mRNA; TcR α was not expressed, whereas only a truncated form of TcR β mRNA was present. These results indicate that the IL‐A29 and CC15 antibodies define a unique population of CD4−CD8−, γ/δ T cells. Copyright © 1990 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim
Authors & Co-Authors
Clevers, Hans C.
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Netherlands, Utrecht
University Medical Center Utrecht
MacHugh, Niall D.
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Bensaid, Albert M.
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Dunlap, Sabrina
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Baldwin, Cynthia L.
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
United States, Columbus
The Ohio State University
Kaushal, Anita
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Iams, Keith Paul
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Howard, Christopher John
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Ivan Morrison, W.
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Statistics
Citations: 200
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1002/eji.1830200415
e-ISSN:
15214141
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study