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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
In vitro conservation of oil palm somatic embryos for 20 years on a hormone-free culture medium: Characteristics of the embryogenic cultures, derived plantlets and adult palms
Plant Cell Reports, Volume 29, No. 1, Year 2010
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Description
This study was conducted over a period of 20 years, to assess the problems involved in developing subcultures over a very long period, of oil palm (Elaeis guineensis Jacq.) somatic embryos which were maintained in vitro on a Murashige and Skoog mineral-based culture medium, without growth regulators. Analysis of the proliferation rate of the embryogenic cultures, along with the survivability of the regenerated plantlets after their transfer into soil and of the flowering of the derived adult palms has been conducted for cultures maintained in vitro during 1 to 20 years. From the ninth year of maintenance, the tissue quality of the somatic embryos gradually began to decline. However, after more than 20 years, 30% of the 20 clones tested still continued to proliferate satisfactorily on the same maintenance medium, keeping their multiplication potential intact. Even though a depressive effect of the age of the lines has been observed on the survival capacity of plants under natural conditions, it is noteworthy that among the clones originating from 20-year-old cultures only eight of them (40%) have exhibited the "mantled" floral abnormality. Different hypotheses concerning the origin of the disruptions observed on the in vitro cultures, plantlets and adult palms that occur over a very long period of in vitro conservation are discussed. © Springer-Verlag 2009.
Authors & Co-Authors
Konan, K. Eugene
Cote D'ivoire, Abidjan
Centre National de Recherche Agronomique Abidjan
Durand-Gasselin, Tristan
France, Paris
Cirad
Kouadio, Yatty Justin
Cote D'ivoire, Abidjan
Université Abobo-adjamé
Flori, Albert
France, Paris
Cirad
Rival, Alain
France, Montpellier
Ird Centre de Montpellier
Duval, Yves
France, Montpellier
Ird Centre de Montpellier
Pannetier, Catherine
France, Versailles
Centre de Recherche Île-de-france - Versailles-grignon
Statistics
Citations: 50
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1007/s00299-009-0787-y
ISSN:
07217714