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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Regional dissemination of a trimethoprim-resistance gene cassette via a successful transposable element
PLoS ONE, Volume 7, No. 5, Article e38142, Year 2012
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Description
Background: Antimicrobial resistance is a growing international problem. We observed a 50% increase in the prevalence of trimethoprim resistance among fecal Escherichia coli from healthy Nigerian students between 1998 and 2005, a trend to increase that continued in 2009. Methods and Findings: A PCR-based screen revealed that 131 (43.1%) of isolates obtained in Nigeria in 2005 and 2009 carried integron-borne dfrA cassettes. In the case of 67 (51.1%) of these isolates, the cassette was a class 1-integron-borne dfrA7 gene, which has been reported at high prevalence from E. coli isolates from other parts of Africa. Complete sequencing of a 27 Kb dfrA7-bearing plasmid from one isolate located the dfrA7 gene within a Tn21-type transposon. The transposon also contained an IS26-derived bla/sul/str element, encoding resistance to β-lactams, sulphonamides and streptomycin, and mercury resistance genes. Although the plasmid backbone was only found in 12 (5.8%) of trimethoprim-resistant isolates, dfrA7 and other transposon-borne genes were detected in 14 (16.3%) and 32 (26.3%) of trimethoprim resistant isolates collected in Nigeria in 2005 and 2009, respectively. Additionally, 37 (19.3%) of trimethoprim-resistant E. coli isolates collected between 2006 and 2008 from Ghana were positive for the dfrA7 and a transposon marker, but only 4 (2.1%) harbored the plasmid backbone. Conclusions: Our data point to transposition as a principal mechanism for disseminating dfrA7 among E. coli from Nigeria and Ghana. On-going intensive use of the affordable broad-spectrum antibacterials is likely to promote selective success of a highly prevalent transposable element in West Africa. © 2012 Labar et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3364232/bin/pone.0038142.s001.pptx
https://efashare.b-cdn.net/share/pmc/articles/PMC3364232/bin/pone.0038142.s002.doc
https://efashare.b-cdn.net/share/pmc/articles/PMC3364232/bin/pone.0038142.s003.doc
https://efashare.b-cdn.net/share/pmc/articles/PMC3364232/bin/pone.0038142.s004.doc
Authors & Co-Authors
Labar, Amy S.
United States, Haverford
Haverford College
Millman, Jennifer S.
United States, Haverford
Haverford College
Ruebush, Ellen
United States, Haverford
Haverford College
Opintan, Japheth
Ghana, Accra
University of Ghana
Bishar, Rima A.
United States, Haverford
Haverford College
Aboderin, Aaron O.
Nigeria, Ife
Obafemi Awolowo University
Newman, Mercy Jemima
Ghana, Accra
University of Ghana
Lamikanra, Adebayo B.
Nigeria, Ife
Obafemi Awolowo University
Okeke, Iruka N.
United States, Haverford
Haverford College
Statistics
Citations: 44
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1371/journal.pone.0038142
e-ISSN:
19326203
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study
Study Locations
Multi-countries
Ghana
Nigeria