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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Exploring glyoxalase 1 expression in prostate cancer tissues: Targeting the enzyme by ethyl pyruvate defangs some malignancy-associated properties
Prostate, Volume 74, No. 1, Year 2014
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Description
BACKGROUND The glyoxalase (GLO)1 is part of a ubiquitous detoxification system in the glycolytic pathway of normal and tumor cells. It protects against cellular damage caused by cytotoxic metabolites. METHODS Aiming at exploring the role of GLO1 in prostate cancer, we evaluated and targeted the expression of GLO1 in prostate cancer tissues and cell lines and analyzed its correlation with grading systems and tumor growth indices. RESULTS Immunohistochemical studies on 37 prostate cancer specimens revealed a positive correlation between Helpap-grading and the cytoplasmic (P = 0.002)/nuclear (P = 0.006) GLO1 level. A positive correlation between Ki-67 proliferation marker and the cytoplasmic GLO1 (P = 0.006) was evident. Furthermore, the highest GLO1 level was detected in the androgen-sensitive LNCaP compared to the androgen-independent Du-145 and PC-3 prostate cell lines and the breast cancer cell MCF-7, both at protein and mRNA level. Treating cancer cells with ethyl pyruvate was found to defang some malignancy-associated properties of cancer cells including proliferation, invasion and anchorage-independent growth. In vitro results revealed that the potency of ethyl pyruvate is increased when cells are metabolically activated by growth stimulators, for example, by fetal calf serum, dihydrotestosterone, tumor growth factor-β1 and leptin. CONCLUSIONS The positive correlation of GLO1 expression level in prostate cancer tissues with the pathological grade and proliferation rate may assign GLO1 as a risk factor for prostate cancer development and progression. Furthermore, our data indicate that inhibitors of GLO1 might be useful to decelerate the cancer cell growth by a novel therapeutic approach that we may call "induced metabolic catastrophe." © 2013 Wiley Periodicals, Inc.
Authors & Co-Authors
Baunacke, Martin
Germany, Leipzig
Universität Leipzig
Horn, Lars Christian
Germany, Leipzig
Universität Leipzig
Trettner, Susanne
Germany, Leipzig
Universität Leipzig
Engel, Kathrin M.
Germany, Leipzig
Universität Leipzig
Hemdan, Nasr Y.A.
Germany, Leipzig
Universität Leipzig
Egypt, Alexandria
Faculty of Science
Wiechmann, Volker
Germany, Leipzig
Klinikum St. Georg Leipzig
Stolzenburg, Jens Uwe
Germany, Leipzig
Universität Leipzig
Bigl, Marina
Germany, Leipzig
Universität Leipzig
Birkenmeier, Gerd
Germany, Leipzig
Universität Leipzig
Statistics
Citations: 38
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1002/pros.22728
ISSN:
02704137
e-ISSN:
10970045
Research Areas
Cancer
Noncommunicable Diseases