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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Class π glutathione S‐transferase from pig lung: Purification, biochemical characterization, primary structure and crystallization
European Journal of Biochemistry, Volume 196, No. 3, Year 1991
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Description
A cytosolic glutathione S‐transferase from pig lung was purified 210‐fold to apparent homogeneity. The enzyme was classified as a class φ isoenzyme on the basis of its physical and chemical properties. It is homodimeric with a subunit Mr of 23 500, has a pl of 7.2, and shows a high specific activity towards ethacrynic acid. The glutathione analogues, S‐hexylglutathione and glutathione sulfonate, were strong reversible inhibitors. The enzyme's primary structure, established entirely by protein chemical methods, consists of 203 amino acids and is highly similar (82–84% residue identity) to the rat and human class φ isoenzymes. Furthermore, there was no evidence of microheterogeneity or post‐translational modifications. Each subunit contains a highly reactive cysteine residue, the modification of which leads to enzyme inactivation. None of the cysteine residues in the pig enzyme appear to form intramolecular disulfide bonds. Single crystals of the glutathione‐S‐transferase‐glutathione‐sulfonate complex were obtained by the hanging‐drop method of vapour diffusion from poly(ethylene glycol) 4000 solutions. The crystals belong to the orthorhombic space group P212121 with unit cell dimensions of a= 10.125 nm, b= 8.253 nm and c= 5.428 nm and diffract to better than 0.22 nm. Copyright © 1991, Wiley Blackwell. All rights reserved
Authors & Co-Authors
Dirr, Heini W.
Germany, Planegg
Max-planck-institut Für Biochemie
South Africa, Johannesburg
University of Johannesburg
Mann, Karlheinz
Germany, Planegg
Max-planck-institut Für Biochemie
Huber, Robert
Germany, Planegg
Max-planck-institut Für Biochemie
Ladenstein, Rudolf
Germany, Planegg
Max-planck-institut Für Biochemie
Reinemer, Peter
Germany, Planegg
Max-planck-institut Für Biochemie
Statistics
Citations: 56
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1111/j.1432-1033.1991.tb15867.x
ISSN:
00142956
e-ISSN:
14321033