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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Use of massively parallel pyrosequencing to evaluate the diversity of and selection on plasmodium falciparum csp T-cell epitopes in Lilongwe, Malawi
Journal of Infectious Diseases, Volume 206, No. 4, Year 2012
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Description
The development of an effective malaria vaccine has been hampered by the genetic diversity of commonly used target antigens. This diversity has led to concerns about allele-specific immunity limiting the effectiveness of vaccines. Despite extensive genetic diversity of circumsporozoite protein (CS), the most successful malaria vaccine is RTS/S, a monovalent CS vaccine. By use of massively parallel pyrosequencing, we evaluated the diversity of CS haplotypes across the T-cell epitopes in parasites from Lilongwe, Malawi. We identified 57 unique parasite haplotypes from 100 participants. By use of ecological and molecular indexes of diversity, we saw no difference in the diversity of CS haplotypes between adults and children. We saw evidence of weak variant-specific selection within this region of CS, suggesting naturally acquired immunity does induce variant-specific selection on CS. Therefore, the impact of CS vaccines on variant frequencies with widespread implementation of vaccination requires further study. © The Author 2012.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3491736/bin/supp_206_4_580__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3491736/bin/supp_jis329_jis329supp.pdf
Authors & Co-Authors
Bailey, Jeffrey A.
United States, Boston
University of Massachusetts System
Mvalo, Tisungane
Malawi, Lilongwe
Unc Project-malawi
Aragam, Nagesh
United States, Chapel Hill
Unc School of Medicine
Weiser, Matthew
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Congdon, Seth
United States, Chapel Hill
Unc School of Medicine
Kamwendo, Deborah D.
Malawi, Lilongwe
Unc Project-malawi
Martinson, Francis E.A.
Malawi, Lilongwe
Unc Project-malawi
Hoffman, Irving F.
United States, Chapel Hill
Unc School of Medicine
Meshnick, Steven Richard
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Juliano, Jonathan J.
United States, Chapel Hill
Unc School of Medicine
Statistics
Citations: 61
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1093/infdis/jis329
ISSN:
00221899
Research Areas
Genetics And Genomics
Infectious Diseases
Maternal And Child Health
Study Locations
Malawi