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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Comparative analyses of the antiviral activities of IgG and IgA antibodies to influenza A virus M2 protein
Viruses, Volume 12, No. 7, Article 780, Year 2020
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Description
Abstract: The influenza A virus (IAV) matrix-2 (M2) protein is an antigenically conserved viral envelope protein that plays an important role in virus budding together with another envelope protein, hemagglutinin (HA). An M2-specific mouse monoclonal IgG antibody, rM2ss23, which binds to the ectodomain of the M2 protein, has been shown to be a non-neutralizing antibody, but inhibits plaque formation of IAV strains. In this study, we generated chimeric rM2ss23 (ch-rM2ss23) IgG and IgA antibodies with the same variable region and compared their antiviral activities. Using gel chromatography, ch-rM2ss23 IgA were divided into three antibody subsets: monomeric IgA (m-IgA), dimeric IgA (d-IgA), and trimeric and tetrameric IgA (t/q-IgA). We found that t/q-IgA had a significantly higher capacity to reduce the plaque size of IAVs than IgG and m-IgA, most likely due to the decreased number of progeny virus particles produced from infected cells. Interestingly, HA-M2 colocalization was remarkably reduced on the infected cell surface in the presence of ch-rM2ss23 antibodies. These results indicate that anti-M2 polymeric IgA restricts IAV budding more efficiently than IgG and suggest a role of anti-M2 IgA in cross-protective immunity to IAVs. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Immunity
Authors & Co-Authors
Okuya, Kosuke
Japan, Sapporo
Hokkaido University
Eguchi, Nao
Japan, Sapporo
Hokkaido University
Manzoor, Rashid
Japan, Sapporo
Hokkaido University
Yoshida, Reiko
Japan, Sapporo
Hokkaido University
Saito, Shinji
Japan, Tokyo
National Institute of Infectious Diseases
Suzuki, Tadaki
Japan, Tokyo
National Institute of Infectious Diseases
Sasaki, Michihito
Japan, Sapporo
Hokkaido University
Saito, Takeshi
Japan, Sapporo
Hokkaido University
Kida, Yurie
Japan, Sapporo
Hokkaido University
Mori-Kajihara, Akina
Japan, Sapporo
Hokkaido University
Miyamoto, Hiroko
Japan, Sapporo
Hokkaido University
Ichii, Osamu
Japan, Sapporo
Faculty of Veterinary Medicine
Kajihara, Masahiro
Japan, Sapporo
Hokkaido University
Higashi, Hideaki
Japan, Sapporo
Hokkaido University
Takada, Ayato
Japan, Sapporo
Hokkaido University
Statistics
Citations: 6
Authors: 15
Affiliations: 3
Identifiers
Doi:
10.3390/v12070780
ISSN:
19994915