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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Expression of large tenascin-C splice variants by hepatic stellate cells/myofibroblasts in chronic hepatitis C
Journal of Hepatology, Volume 46, No. 4, Year 2007
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Description
Background/Aims: Earlier studies have suggested involvement of tenascin-C (TN-C) in liver fibrosis. Here, we examined expression of TN-C variants and types of alternatively spliced fibronectin-type III (FNIII) repeats in chronic hepatitis. Methods: Using three monoclonal antibodies against TN-C variants, immunohistochemical staining was performed and the correlation with histological parameters of chronic hepatitis C was examined. The cellular source was also determined and variant expression and their types were tested using isolated rat hepatic stellate cells (HSCs), liver myofibroblasts, and/or LI90 cells. Results: Large variants were not expressed in normal liver, but were up-regulated in chronic hepatitis, especially at sites of interface hepatitis and confluent necrosis, showing stronger correlations between staining intensity and these than with other parameters or fibrosis. TN-C deposition was closely correlated with increase in the number of α-smooth muscle actin-positive cells, i.e. activated HSCs/myofibroblasts, and in situ hybridization showed TN-C mRNA signals in the cells. Activated HSCs and myofibroblasts in culture highly expressed large variants of TN-C. In LI90 cells, sequencing of large variants revealed that the FNIII repeats D and A1/A4, followed by B, were preferentially included. Conclusions: TN-C and its variants are produced by HSCs/myofibroblasts, suggesting important roles in liver fibrogenesis. © 2006 European Association for the Study of the Liver.
Authors & Co-Authors
El-Karef, Amro Awad
Japan, Tsu
Mie University Graduate School of Medicine
Kaito, Masahiko
Japan, Tsu
Mie University Graduate School of Medicine
Inada, Hiroyasu
Japan, Tsu
Mie University Graduate School of Medicine
Kawada, Norifumi
Japan, Osaka
Osaka Metropolitan University Graduate School of Medicine
Imanaka-Yoshida, Kyoko
Japan, Tsu
Mie University Graduate School of Medicine
Yoshida, Toshimichi
Japan, Tsu
Mie University Graduate School of Medicine
Statistics
Citations: 32
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1016/j.jhep.2006.10.011
ISSN:
01688278
Research Areas
Genetics And Genomics
Infectious Diseases