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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Loop-mediated isothermal amplification for rapid detection of the causal agents of cassava brown streak disease
Journal of Virological Methods, Volume 191, No. 2, Year 2013
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Description
The causal agents of cassava brown streak disease have recently been identified as Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Primers have been developed for rapid detection of these viruses by reverse transcription loop-mediated isothermal amplification (RT-LAMP). Performance of the RT-LAMP assays compared favourably with published RT-PCR and real-time RT-PCR methods. Furthermore, amplification by RT-LAMP is completed in 40. min and does not require thermal cycling equipment. Modification of the RT-LAMP reactions to use labelled primers allowed rapid detection of amplification products using lateral flow devices containing antibodies specific to the incorporated labels, avoiding the need for fluorescence detection or gel electrophoresis. © 2012.
Authors & Co-Authors
Tomlinson, Jenny A.
United Kingdom, York
Fera Science Limited
Ostoja-Starzewska, S.
United Kingdom, York
Fera Science Limited
Adams, Ian Patrick
United Kingdom, York
Fera Science Limited
Miano, Douglas Watuku
Kenya, Nairobi
Kenya Agricultural Research Institute
Abidrabo, Phillip
Uganda, Kampala
National Crops Resources Research Institute
Uganda, Kampala
Makerere University
Kinyua, Zakary M.
Kenya, Nairobi
Kenya Agricultural Research Institute
Alicai, Titus
Uganda, Kampala
National Crops Resources Research Institute
Dickinson, Matthew J.
United Kingdom, Nottingham
University of Nottingham
Peters, D.
Kenya, Nairobi
Africa Regional Office
Boonham, Neil
United Kingdom, York
Fera Science Limited
Smith, Julian J.
United Kingdom, York
Fera Science Limited
Statistics
Citations: 48
Authors: 11
Affiliations: 6
Identifiers
Doi:
10.1016/j.jviromet.2012.07.015
ISSN:
01660934
e-ISSN:
18790984