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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Sperm DNA fragmentation and oxidation are independent of malondialdheyde
Reproductive Biology and Endocrinology, Volume 9, Article 47, Year 2011
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Description
Background: There is clinical evidence to show that sperm DNA damage could be a marker of sperm quality and extensive data exist on the relationship between DNA damage and male fertility status. Detecting such damage in sperm could provide new elements besides semen parameters in diagnosing male infertility. We aimed to assess sperm DNA fragmentation and oxidation and to study the association between these two markers, routine semen parameters and malondialdehyde formation.Methods: Semen samples from 55 men attending the Histology-Embryology Laboratory of Sfax Faculty of Medicine, Tunisia, for semen investigations were analysed for sperm DNA fragmentation and oxidation using flow cytometry. The Sperm was also assessed spectrophotometrically for malondialdehyde formation.Results: Within the studied group, 21 patients were nonasthenozoospermic (sperm motility ≥ 50%) and 34 patients were considered asthenozoospermic (sperm motility < 50%). A positive correlation was found between sperm DNA fragmentation and oxidation (p = 0.01; r = 0.33). We also found a negative correlation between sperm DNA fragmentation and some sperm parameters: total motility (p = 0.001; r = -0.43), rapid progressive motility (type a motility) (p = 0.04; r = -0.27), slow progressive motility (type b motility) (p = 0.03; r = -0.28), and vitality (p < 0.001; r = -0.65). Sperm DNA fragmentation was positively correlated with coiled tail (p = 0.01; r = 0.34). The two parameters that were found to be correlated with oxidative DNA damage were leucocytes concentrations (p = 0.01; r = 0.38) and broken neck (p = 0.02; r = 0.29). Sperm MDA levels were negatively correlated with sperm concentration (p < 0.001; r = -0.57), total motility (p = 0.01; r = -0.35) and type a motility (p = 0.03; r = -0.32); but not correlated with DNA fragmentation and DNA oxidation.Conclusions: Our results support the evidence that oxidative stress plays a key role in inducing DNA damage; but nuclear alterations and malondialdehyde don't seem to be synchronous. © 2011 Zribi et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Zribi, Nassira
Tunisia, Sfax
Faculty of Medicine of Sfax
Chakroun, Nozha Feki
Tunisia, Sfax
Faculty of Medicine of Sfax
Elleuch, Henda
Tunisia, Sfax
University of Sfax
Ben Abdallah, Fatma
Tunisia, Sfax
Faculty of Medicine of Sfax
Ben Hamida, Afifa Sellami
Tunisia, Sfax
Faculty of Medicine of Sfax
Gargouri, Jalel
Tunisia, Sfax
University of Sfax
Fakhfakh, Faiza
Tunisia, Sfax
Faculty of Medicine of Sfax
Ammar-Keskes, Leila
Tunisia, Sfax
Faculty of Medicine of Sfax
Statistics
Citations: 89
Authors: 8
Affiliations: 2
Identifiers
Doi:
10.1186/1477-7827-9-47
e-ISSN:
14777827
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Sexual And Reproductive Health
Study Locations
Tunisia
Participants Gender
Male