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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Oligonucleotide fingerprinting detects genetic diversity among Ascochyta rabiei isolates from a single chickpea field in Tunisia
Current Genetics, Volume 26, No. 3, Year 1994
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Description
Fifty isolates of Ascochyta rabiei (Pass.) Labr. were hierarchically sampled from four well-separated locations of a single chickpea field in Beja (Tunisia), and single-spored. DNA was isolated from in-vitro-grown mycelia, digested with HinfI or RsaI, and hybridized to a set of synthetic oligonucleotides complementary to simple repetitive sequences. According to the fingerprint patterns derived from the probes (CA)8, (CAA)5, (CAT)5 and (GATA)4, 12 different fungal haplotypes were found at various frequencies within the investigated field. Seven haplotypes were confined to one location only, four occurred at two, one at three, and none at all four locations. Most of the genetic variability originated from diversity within, rather than between, locations. In some cases, more than one haplotype was isolated from the same lesion of a single host plant. Genetic distances between isolates, as calculated from band-sharing data, varied between 0.05 and 0.22. Relatedness between the different haplotypes was evaluated by cluster analysis using UPGMA. © 1994 Springer-Verlag.
Authors & Co-Authors
Morjane, Hichem
Germany, Frankfurt am Main
Goethe-universität Frankfurt am Main
Tunisia, Tunis
University of Carthage, Institut National Agronomique de Tunisie
Geistlinger, Joerg
Germany, Frankfurt am Main
Goethe-universität Frankfurt am Main
Harrabi, Moncef
Tunisia, Tunis
University of Carthage, Institut National Agronomique de Tunisie
Weising, Kurt
Germany, Frankfurt am Main
Goethe-universität Frankfurt am Main
Kahl, Günter
Germany, Frankfurt am Main
Goethe-universität Frankfurt am Main
Statistics
Citations: 44
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1007/BF00309547
ISSN:
01728083
e-ISSN:
14320983
Research Areas
Genetics And Genomics
Study Locations
Tunisia