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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Detection of Hepatitis B Virus DNA Directly in Human Serum by a Simplified Molecular Hybridization Test: Comparison to HBeAg/ Anti‐HBe Status in HBsAg Carriers
Hepatology, Volume 3, No. 3, Year 1983
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Description
A simple, direct molecular hybridization test was employed to detect hepatitis B virus (HBV) DNA sequences in human serum. In 61 HBsAg carriers, many with HBV‐related diseases (chronic persistent hepatitis, chronic active hepatitis, or posthepatitic cirrhosis), 28/28 (100%) who were HBeAg* and 16/32 (50%) who were anti‐HBe+ had HBV DNA sequences in their serum. Among 22 South African black patients with hepatocellular carcinoma, 7 (32%) had detectable HBV DNA in their serum but at reduced levels when compared to HBsAg carriers without hepatocellular carcinoma, suggesting that viral replication is suppressed or inactive in many hepatocellular carcinoma patients. Hybridization analysis also distinguished carriers with high, moderate, or low amounts of HBV DNA in serum. Ten to 20% of HBsAg+/HBeAg+ carriers showed high serum levels of HBV DNA but, surprisingly, a similar percentage of HBsAg+/anti‐HBe+ carriers also showed relatively high serum levels of HBV DNA. Five patients who had undergone immunosuppression therapy and most of whom were on chronic hemodialysis had very high serum levels of HBV DNA, in the range observed during acute HBV infection. By epidemiologic analysis, two of these individuals were implicated in transmission of hepatitis to other hemodialysis patients, paramedical personnel, or intimate family contacts. Serum HBV DNA hybridization analysis identifies carriers with high serum levels of HBV irrespective of HBeAg/anti‐HBe status and may define individuals with potentially high risk of transmitting infection to their immediate contacts. Copyright © 1983 American Association for the Study of Liver Diseases
Authors & Co-Authors
Lieberman, Harvey M.
United States, New York
Albert Einstein College of Medicine of Yeshiva University
United States, Iowa City
University of Iowa
South Africa, Johannesburg
School of Clinical Medicine
Greece, Athens
Hippokration General Hospital
Labrecque, Douglas R.
United States, New York
Albert Einstein College of Medicine of Yeshiva University
United States, Iowa City
University of Iowa
South Africa, Johannesburg
School of Clinical Medicine
Greece, Athens
Hippokration General Hospital
Kew, Michael Charles
United States, New York
Albert Einstein College of Medicine of Yeshiva University
United States, Iowa City
University of Iowa
South Africa, Johannesburg
School of Clinical Medicine
Greece, Athens
Hippokration General Hospital
Hadziyannis, Stephanos J.
United States, New York
Albert Einstein College of Medicine of Yeshiva University
United States, Iowa City
University of Iowa
South Africa, Johannesburg
School of Clinical Medicine
Greece, Athens
Hippokration General Hospital
Shafritz, David A.
United States, New York
Albert Einstein College of Medicine of Yeshiva University
United States, Iowa City
University of Iowa
South Africa, Johannesburg
School of Clinical Medicine
Greece, Athens
Hippokration General Hospital
Statistics
Citations: 227
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1002/hep.1840030302
ISSN:
02709139
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases