MOLECULAR DETECTION OF HELICOBACTER PYLORI BY REAL TIME -PCR IN GASTROINTESTINAL DISEASES PATIENTS

Helicobacter pylori (H. pylori) is one of the most common infection worldwide and is associated with gastric disorders. It has been considered one of the main risk factors that cause gastric cancer. H.pylori is genetically unstable and this reflected on its virulence factors and type of diseases they causes. To achieve the aim of the study in the detection H.pylori infection by invasive method were used, biopsy samples. Also, to assess the relationship between each ofAge group and Gender with H. pylori infection. The current study protocol was approved by the Ethics and Research Committees of Kirkuk Health Directorate and all patients gave informed. This study was carried out in Biology Department Laboratories, College of Science, Kirkuk University and in Wahj-DNA Office, Baghdad. The samples were collected during the period November 2017 to March 2019 from 50 patients with gastritis in the endoscopy unit to Azadi Hospital, Kirkuk, Medical city Hospital and Al-Arabi private Hospital in Baghdad). Depending on the endoscopic diagnosis (O.G.D) findings of studied patients were gastritis in 26(52%) patients, Gastric ulcer in 19(38%) patients, abdominal pain without ulcer in 4(8%) patients and gastric cancer in 1(2%) patients. Rapid urease test was used for primary direct identification of H. pylori from gastric tissue biopsy sample. The results were shown that H. pylori identification percentage by using urease test was 80%(40/50) and by by RT-PCR according to the manufacturer’s and the percentage results have shown at 92%(46/50). Also showed that there are closely relatedness between the infection and the age group of 41 to 50 years 40% comparing with other age group and infected in the male category more than female at rate 64%. However, the results of current study showed significant according chi-square and odd ratio the p-value <0.3377 between RUT and RT-RCR, determine the degree of agreement between them.