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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Lack of insertional-deletional polymorphism in a collection of Mycobacterium ulcerans isolates from Ghanaian Buruli ulcer patients
Journal of Clinical Microbiology, Volume 47, No. 11, Year 2009
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Description
Mycobacterium ulcerans causes the devastating infectious skin disease Buruli ulcer and has a monomorphic population structure. The resolution of conventional genetic fingerprinting methods is therefore not sufficient for microepidemiological studies aiming to characterize transmission pathways. In a previous comparative genomic hybridization analysis with a microarray covering part of the M. ulcerans genome, we have found extensive insertional-deletional sequence polymorphisms among M. ulcerans isolates of diverse geographic origins that allowed us to distinguish between strains coming from different continents. Since large numbers of insertion sequences are spread over the genome of African M. ulcerans strains, we reasoned that these may drive large sequence polymorphisms in otherwise clonal local mycobacterial populations. In this study, we used a printed DNA microarray covering the whole genome of the Ghanaian M. ulcerans reference strain Agy99 for comparative genomic hybridization. The assay identified multiple regions of difference when DNA of a Japanese M. ulcerans strain was analyzed. In contrast, not a single insertional-deletional genomic variation was found within a panel of disease isolates coming from an area of Ghana where Buruli ulcer is endemic. These results indicate that, despite the expectations deduced from other mycobacterial pathogens, only analyses of single nucleotide polymorphisms will have the potential to differentiate local populations of M. ulcerans. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Käser, Michael
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Gutmann, Oliver
Switzerland, Basel
F. Hoffmann-la Roche ag
Hauser, Julia
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Stinear, Timothy Paul
Australia, Clayton
Monash University
Cole, Stewart Thomas
Switzerland, Lausanne
École Polytechnique Fédérale de Lausanne
Yeboah-Manu, Dorothy
Ghana, Accra
Noguchi Memorial Institute for Medical Research
Dernick, Gregor
Switzerland, Basel
F. Hoffmann-la Roche ag
Certa, U.
Switzerland, Basel
F. Hoffmann-la Roche ag
Pluschke, Gerd
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Statistics
Citations: 16
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1128/JCM.00760-09
ISSN:
00951137
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study
Study Locations
Multi-countries
Ghana