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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Expression strategy of densonucleosis virus from Mythimna loreyi
Virology, Volume 320, No. 1, Year 2004
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Description
The genome of Mythimna loreyi densovirus (MlDNV) was cloned into the pEMBL(19)+ vector. This clone was infectious upon transfection, both in LD cells and larvae. The genome possessed ITRs of 543 nucleotides of which the distal 126 nucleotides could form a hairpin. The nonstructural (NS) and structural (VP) genes were located on the 5′-halves of the complementary strands and their transcripts started 27 nts downstream of the ITRs. These transcripts had an overlap of 57 nucleotides in middle of the genome. The NS cassette consisted of three genes with NS1 and the overlapping NS2 downstream of NS3. The NS3 gene was spliced out from a fraction of the NS transcripts to allow leaky scanning translation of the downstream bicistronic NS1 and NS2 genes. The four VPs were similarly generated by leaky scanning translation of unspliced mRNA. The 5′-untranslated region of the VP transcript was only seven nucleotides long. © 2004 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Fédière, G.
Canada, Quebec
Université Laval
Egypt, Giza
Cairo University
El-Far, Mohamed
Canada, Quebec
Université Laval
Li, Yi
Canada, Quebec
Université Laval
Bergoin, Max
France, Montpellier
Université de Montpellier
Tijssen, Peter
Canada, Quebec
Université Laval
Statistics
Citations: 42
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1016/j.virol.2003.11.033
ISSN:
00426822
Research Areas
Genetics And Genomics